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A Cross-Sectional Study on Gastrointestinal Parasites in Backyard Poultry in Krishna District, Andhra Pradesh, INDIA

Anupama Bandi Malakondaiah Pattipati Sreedevi Chennuru Ravi Kumar Pentela Satheesh Kokila
46-60
DOI- http://dx.doi.org/10.5455/ijlr.20190805120338

A total of 1447 desi birds’ samples (faeces, gastrointestinal tracts) collected from various villages in Krishna district, Andhra Pradesh were examined from July 2015 to June 2016 to determine the prevalence of gastrointestinal (GI) parasites. The overall prevalence was 74.22 per cent. Mixed infection was more frequent (70.39 %) and cestodes were the common parasites in all infected birds. No trematode parasite was identified. The species identified includes Amoebotaenia sphenoides, Cotugnia digonopora, Davainea proglottina, Hymenolepis carioca, Raillietina cesticillus, R. echinobothridia and R. tetragona in cestodes, Ascaridia galli, Capillaria spp., Heterakis gallinarum, Strongyloides avium, Subulura brumpti, Tetrameres mohtedai and Dispharynx spiralis in nematodes and Eimeria spp. in protozoa. The prevalence of infection was increased with advance in age. Statistically there was no significant relationship between the prevalence of infection and age, sex and seasons (P>0.05). Cotugnia digonopora was the highest prevalent parasite. Dispharynx spiralis was exclusively found in chicks and in summer. Tetrameres mohtedai was exclusively identified in female bird.


Keywords : Age Wise Desi Birds Gastrointestinal Parasites Prevalence Sex Wise and Season Wise

How to cite: Bandi, A., Pattipati, M., Chennuru, S., Pentela, R., & Kokila, S. (2020). A cross-sectional study on gastrointestinal parasites in backyard poultry in Krishna district, Andhra Pradesh, India. International Journal of Livestock Research, 10(2), 46-60. doi: 10.5455/ijlr.20190805120338

Introduction

Poultry farming is one of the rapidly growing profitable animal production enterprises (Obiora, 1992). India is the third-largest egg producer and the fourth largest chicken-producer (Kotaiah, 2016). Poultry farming under backyard system is as old as its civilization and contributes nearly 30.0 per cent of Indian egg production (Singh et al., 2009). Investments in backyard poultry farming can stimulate the growth of the economy as their eggs and meat fetches a much higher price than that from commercial poultry hence, the government of India included it in its eleventh five-year plan (2007-2012) (Pica-Ciamarra and Dhawan, 2009). Backyard poultry rearing had a significant impact towards socio-economic development of the rural women folk in generating more income for leading their families happy (Rayala Reddy et al., 2017). The rural women are primarily agricultural labourers keeping small size backyard poultry flock as secondary occupation with marginal land holding, low annual family income, low annual income from backyard poultry (21.10% of annual family income) (Bharti et al., 2018).

However, due to their scavenging habits backyard poultry are more prone to variety of infectious diseases and parasitic infections are among the one that are considered as major constraint to the economy of farming through reduced egg production and weight gain. Hence, there is a need for systematic observation and documentation of the occurrence of parasites in different regions in different age groups, sex and seasons to formulate and undertake appropriate control strategies. Globally, the prevalence of GI parasites in desi birds has been reported from different countries (Percy et al., 2012) including India (Puttalakshmamma et al., 2008; Katoch et al., 2012). Yet, it is crucial to carry out up-to-date epidemiological studies considering the dynamics of parasitic infections. However, reports on prevalence of endoparasites in backyard desi fowl of Andhra Pradesh are scanty and is restricted to one particular region (Sreedevi et al., 2016). In view of this, the present study is aimed to record the prevalence of GI parasites in backyard poultry in Krishna district, Andhra Pradesh, India.

Materials and Methods

Study Area

The study area Krishna district occupies an area of approximately 8727 km2. It is located at an altitude of 97-100 meters above sea level. Its latitude is 16.1 and longitude is 81.1. The climatic conditions of the district are extremely hot summer and moderately hot winter and may be classified as tropical. The annual rainfall in the region is about 1028 mm and is contributed to by the Southwest monsoon. The mean annual minimum and maximum temperature is 18.7 °C and 39.8 °C respectively. The majority of the people in urban areas of Krishna district are engaged in trade and commerce.

Sample Collection

A total of 1447 desi birds’ samples (745 faecal samples and 702 Gastrointestinal tracts (GITs) were collected from Kesarapalli, Gannavaram, Bapulapadu, Srinarsannapalem, Bathulavarigudem, Unguturu, Ravicherla and Indupalli villages in Krishna district, Andhra Pradesh for a period of one year from July 2015 to June 2016 to know the prevalence of gastrointestinal parasites. GITs were collected from freshly slaughtered birds at local poultry shops of the said villages. The birds were randomly sampled per household with consideration of age, sex and season. The birds were divided in to three groups as chicks (up to 8 wk), growers (9 to 16 wk) and adults (above 16 wk). Depending on climatic conditions prevailing in Andhra Pradesh the whole year was divided in to three seasons viz. summer (March to June), monsoon or rainy (Jun to October) and winter (November to February). A cross sectional survey was done to pertain the information regarding the dynamics of flock size, number of eggs laid, percentage hatch, number successfully brooded, number that attained adulthood among different age group. The average size of flocks in different villages was 5-15 birds.

Processing of Samples

Faecal samples were examined by direct smear and concentration by floatation with 33.3 per cent zinc sulphate solution and sedimentation methods. Samples that were positive for coccidian oocysts were kept in 2.5 per cent potassium dichromate solution for specific identification. Each GIT was incised longitudinally and was immersed in luke warm water for the release of worms that were embedded in intestinal mucosa. Intestinal scrapings were also examined for the presence of tape worm heads, small tape worm and developmental stages of coccidian parasites. Any visible worms were collected and were fixed in fixatives like 10% formalin (round worms) and Boun’s fluid (cestodes) for further species-specific processing as per the standard procedure. The intestinal contents were washed into separate petri dishes and were centrifuged and examined for the presence of eggs, oocysts and larval stages of parasites. The identification of eggs, cysts and adult parasites was carried out as per the description of Soulsby (1982) and Saif et al. (2008). Oocysts of Eimeria spp. were identified based on sporulation time and micrometry.

Statistical Analysis

Data obtained was classified according to age, sex and season and was analyzed as per standard statistical techniques (Petrie and Watson, 2013). Chi-square test of association was used to establish association between the infection status and variables such as age, sex and season of the desi bird.

Results and Discussion

A cross sectional survey on backyard poultry revealed that hens lay between 10-16 eggs prior to natural incubation out of which hatchability varies from 70.0 to 100 per cent based on the number successfully brooded. Depending on season between 60.0 to 80.0 per cent of the hatchlings were successfully brooded.

Overall Prevalence and Species Wise Prevalence of GI Parasites

Out of 1447 birds examined, on faeces and GIT examination, 1074 birds revealed different helminth and protozoa infections giving an overall prevalence of 74.2 per cent of GI parasites in the study area. Examination of GIT of birds revealed presence of parasites in all samples (100%). Examination of intestinal scrapings revealed presence of schizonts of coccidia, Amoebotaenia sphenoides and Davainea proglottina. The infection was more prevalent in Kesarapalli (78.50%) village compared to that of Gannavaram (76.41%), Bapulapadu (75.54%), Srinarsannapalem (75.16%), Bathulavarigudem (73.85%), Unguturu (71.83%), Ravicherla (70.85%) and Indupalli (70.61 %) villages of Krishna district. It was noticed that there was an increase in prevalence over the past 5 years in Gannavaram region wherein the prevalence was 63.21 per cent in 2014 (Sreedevi et al., 2016) warranting control measures on parasitic infections. Similarly, Puttalakshmamma et al. (2008), Katoch et al. (2012) and Sonune et al. (2012) reported 71.0, 72.0 and 72.0 per cent of infection in desi birds in Bangalore, Jammu and Maharashtra respectively. Higher prevalence than in the present study was reported in other Asian country, Bangladesh (91.88%) by Alam et al. (2014). However, lower prevalence of 58.75 per cent was noticed by Hembram et al. (2015) in Odisha. The difference in the prevalence of parasitic infection could be due to the difference in climatic conditions of region, socio-economic conditions of the people, availability of intermediate hosts or adaptability of managemental practices (Percy et al., 2012; Sreedevi et al., 2016).

Out of 1047 infected birds, 29.61 per cent were found to be positive exclusively for cestodes, 60.89 per cent for cestodes and nematodes, 4.46 per cent for cestodes, nematodes and Eimeria spp. and 5.02 percent for cestodes and Eimeria spp. Mixed infection (70.39%) was more common than single infection (29.61%) and cestodes were the common parasites in all infected birds (100%). Similarly, Eshetu et al. (2001) also reported 73.78 per cent prevalence of mixed infection in Ethiopia. A similar pattern of higher prevalence of cestodes over nematodes has also been reported by Puttalakshmamma et al. (2008) in Bangalore (52.2 %) and Sonune et al. (2012) in Maharashtra (52.77%). Contrary to the present finding Katoch et al. (2012) in Jammu (56.66 %), Naphade and Chaudhari (2013) in Maharashtra (40%) and Solanki et al. (2015) in South Gujarath (40.87%) reported high prevalence of nematodes over cestodes. The higher prevalence of cestodes in the present study could be due to frequent exposure of the backyard poultry to intermediate hosts of cestodes and less accessibility to infective stages of nematodes in the environment in addition to immunological and nutritional status of the individuals in a particular geographical area. The per cent prevalence of different parasites identified in the present study was presented in Table 1 and Fig. 1&2. There was a significant (P<0.05) difference in prevalence between species of parasites of infected birds in accordance to the findings of Momin et al. (2014).

Table1: Prevalence of endoparasites in desi birds in different villages of Krishna district, Andhra Pradesh

S. No. Name of the Mandal Name of the Village No. of bird’s samples examined No. of bird’s samples found positive % positive
1 Bapulapadu Bapulapadu 184 139 75.54
Srinarsannapalem 149 112 75.16
2 Gannavaram Gannavaram 212 162 76.41
Kesarapalli 214 168 78.5
3 Nuzvid Bathulavarigudum 153 113 73.85
Ravicherla 199 141 70.85
4 Unguturu Unguturu 142 102 71.83
Indupalli 194 137 70.61
Total   1447 1074 74.22

Fig.1: Prevalence of endoparasites in desi birds in different villages of Krishna district, Andhra Pradesh

Fig. 2: Type of endoparasites in desi birds of Krishna district, Andhra Pradesh

Among all cestode parasites identified Cotugnia digonopora was the highest prevalent parasite (36.8%) which is in agreement with the findings of Butt et al. (2014) who recorded highest (94.5%) prevalence of C. digonopora in Pakistan and Hcarioca was the lowest. However, Bhat et al. (2014) reported lowest (3.3%) prevalence of C. digonopora in North India. In contrast, R. echinobothridia and R. tetragona were the common parasite in Faisalabad (Shah et al., 1999) and in Bengaluru region (Puttalakshmamma et al., 2008).

Among all nematode parasites identified Agalli (30.9 %) was the frequent parasite (Puttalakshmamma et al., 2008; Katoch et al., 2012) in the studied area. However, mortality from Agalli is insignificant, may lead to death of infected bird due to intestinal obstruction. Compared to Agalli infection the prevalence of Hgallinarum (24.5%) was lower, yet its significance lies in its role as a carrier of protozoan parasite Histomonas meleagridis which cause fatal disease in birds. Contrary to our findings Hgallinarum was the highly prevalent nematode in Bhubaneswar (Manaswini, 2007). No trematode parasites were noticed during this study which might be due to lack of accessibility to snails infected with larval stages of trematodes. Present findings were in accordance with previous reports in India (Puttalakshmamma et al., 2008; Sreedevi et al., 2016). Out of four Eimeria spp. identified E. tenella was the most prevalent species. The present results are in accordance with the findings of Sreedevi et al. (2016) who reported E. tenella as prevalent species in desi birds in Gannavaram, one of the study areas.

Description: C:\Users\HP\Desktop\Jesus thesis photos\JESUS paras thesis photos\eggs of parasites\amoeb.JPG

A. Amoebotaenia sphenoides (egg with distinctive granular layer x 400)

Description: C:\Users\HP\Desktop\Jesus thesis photos\JESUS paras thesis photos\eggs of parasites\P1010053.JPG

B. Davainea proglottina (single egg with hexacanth embryo and without egg capsule x 400)

Description: C:\Users\HP\AppData\Local\Microsoft\Windows\INetCache\Content.Word\amoe.jpg

C. Cotugnia digonopora (egg capsule containing single egg x 400)

Description: C:\Users\HP\Desktop\Jesus thesis photos\JESUS paras thesis photos\eggs of parasites\P1010049.JPG

D. Hymenolepis carioca (football shaped embryophore with granular accumulations at the poles x 400)