Association between Polymorphisms of Solute Carrier 27 A1 Gene with Milk Production Traits in Indian Sahiwal and Hariana Cattle
Madhu Tiwari Deepak Sharma Satyendra Pal Singh Rakesh Goel
Vol 7(6), 219-224
This study investigated the status of solute carrier 27A1 (SLC27A1) polymorphism in Indian cattle breeds (n = 102) using PCR-RFLP assay and its association with milk production and reproduction traits. Two region of SLC27A1 gene consisting of exon 3 and exon 4 revealed 261 and 160 bp products, respectively. The digestion of 261 bp product using SacII restriction enzyme (RE) resulted in monomorphic pattern; only GG genotype. The BsaHI/PCR-RFLP assay revealed three types of genotypes, namely, TT (160 bp), CC (106 and 54 bp) and heterozygous CT (160, 106 and 54 bp) genotypes with frequencies 18.62, 59.80 and 21.56 %, respectively. The allelic frequency of C and T alleles were 0.71 and 0.29, respectively. Association study revealed that lactation period (LP), milk yield in 300 days (MY300), calving interval (CI), peak yield (PY) and days to reach peak yield (DRPY) had non-significant difference among all the three genotypes in first and second lactations while, significant difference was (P=0.028) observed for total milk yield (TMY), where TMY of CC genotype was significantly higher than CT and TT genotypes in only first lactation. Results indicated that C allele for SLC27A1 gene can be used as candidate gene for marker assisted selection in Sahiwal and Hariana cattle.
Keywords : SLC27A1 PCR-RFLP Sahiwal Hariana SacII BsaHI
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