Scarcity of green fodder and hot climatic conditions in summer are the major problems faced by the livestock reared for economic purposes. Most of the semi-organized farms in and around Proddatur region of Andhra Pradesh were found to maintain Jersey Cross bred cows and graded Murrah buffaloes in summer by feeding Total Mixed Ration (TMR) or balanced ration along with supplementation of mineral mixture due to the lack of green fodder. Therefore, the present study was undertaken to study the effect of raise in environmental temperature as well as lack of green fodder on the various biochemical parameters of apparently healthy Jersey Cross bred cows and graded Murrah buffaloes in and around Proddatur region of Andhra Pradesh. A total of 29 blood samples were analyzed in both Jersey cross- bred cows and graded murrah buffaloes for various biochemical parameters. The concentration of Total Protein(g/dl), Globulin (g/dl), Urea (mg/dl), Total Cholesterol (mg/dl) and Calcium (mg/dl) were found to be significantly different between Jersey cross bred cows and graded buffaloes(P< 0.05 ). These values obtained can serve as baseline values for Jersey cross bred cows and graded murrah buffaloes of that particular location in summer and also for comparison with the animals maintained during the period of availability of abundant green fodder in kharif season.