The prevalence of Salmonella spp in poultry (meat, egg and cloacal swabs) samples collected in and around Hyderabad by cultural and the Polymerase Chain Reaction (PCR) methods. A total of 150 poultry samples (50 each of meat, egg and cloacal swabs) were tested of which 45 and 50 samples were positive for Salmonella spp by cultural method and PCR assay accounting for 30% and 33.3% respectively. Out of 50 poultry meat samples 17 samples (34%) were positive for Salmonella spp by cultural method, whereas 19(38%) were positive by PCR assay. Out of 50 each of egg shell and cloacal swab samples 15(30%) and 13(26.0%) respectively were positive by cultural method whereas 17(34%) and 14(28%) respectively were positive by PCR assay.