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Detection of Brucella abortus in Cattle and Buffaloes with Spontaneous Abortion from an Organized Dairy Farm

Geeta Devi Leishangthem Vishal Mahajan* Gursimran Filia and Mandeep Singh Bal
Vol 9(3), 164-171
DOI- http://dx.doi.org/10.5455/ijlr.20180910025922

Bovine brucellosis is a contagious, bacterial and economically important disease which causes late term abortions and subsequently retention of placenta in bovines. Bovine brucellosis is endemic in India affecting various domesticated animals viz. buffalo, cattle, goat and sheep. In the present study, 30(17 buffaloes and 13 crossbred cattle) animals from an organized farm with spontaneous abortion, were investigated to detect whether Brucella species was the causative agent from April 2016 to April 2017. Samples collected include fetal stomach content from the aborted fetus, placenta and blood from the aborted animal. Brucella abortus was detected by PCR using primers specific for both genus as well as species from stomach content and placenta. RBPT and c-ELISA were also conducted on the serum samples of the aborted animals. Out of the 30 animals, an overall 15 (50%) animals (11 buffaloes and 4 cattle) were positive for brucellosis by PCR, RBPT and c-ELISA. The present study may provide supportive information regarding the disease situation in Punjab, India.

Keywords : Abortion Brucellosis Buffaloes Cattle PCR ELISA

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