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Detection of Polymorphism in Coding Region of SLC11A1 Gene in Jersey Crossbred Cattle

M. Malarmathi N. Murali R. Saravanan P. Gopu L. Gunaseelan
Vol 9(8), 263-267

SLC11A1 gene (Solute Carrier 11 A1 gene), formerly called as Natural Resistance Associated Macrophage Protein 1 (NRAMP1) plays a critical role in innate immunity and mutation of this gene is responsible for susceptibility to a number of intracellular pathogens. The polymerase chain reaction - restricted fragment length polymorphism (PCR-RFLP) analysis (Pst I) of exon 11 in 52 Jersey crossbred cattle revealed presence of three genotypes CC (231bp), CG (231 bp, 200 bp, 31 bp) and GG (200 bp, 31 bp) confirming C > G transversion in coding region. The genotypic frequencies in pooled samples were estimated as 0.24 (CC), 0.48 (CG) and 0.28 (GG). This polymorphism observed in exon 11 is due to single nucleotide polymorphism (C > G). However, no polymorphic pattern was observed in exonic regions 4-5 and 5-6.

Keywords : Jersey Crossbred Cattle NRAMP1 SNP SLC11A1 Transversion

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