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Detection of Vibrio parahaemolyticus from Marine Crabs Sold in and around Puducherry and Its Public Health Significance

C. Vijayan V. J. Ajaykumar A. Bhattacharya V. Bhanurekka
Vol 8(2), 189-204

Food borne illnesses have major social and economic impacts. The WHO estimates that worldwide food borne and waterborne diarrheal diseases together kill about 2.2 million people annually and in India around 6 per cent of the population. Vibrio parahaemolyticus has recently been recognized as one of the most important food borne pathogens as the leading causal agent of human acute gastro enteritis. This study was conducted to detect the presence of V. parahaemolyuticus from marine crabs sold in puducherry and human stool samples as per the standard procedure. A total of 35 marine crab samples and 50 human stool samples were screened for presence of V. parahaemolyticus. Out of 35 samples, 14 samples (40 per cent) and out of 50 stool specimens, only 2 (4 per cent) were positive for V. parahaemolyticus by conventional methods. This isolates were further subjected to PCR and subjected to biofilm production assay by CRA plate method. Of the 14 isolates, 9 were positive for biofilm production and none of the isolates from human stool samples were positive for biofilm production. The antibiotic resistance profiling of the V. parahaemolyticus isolates does not show high resistance. This can be a major concern as microorganisms can be associated with chronic and recurrent human infections. This study clearly indicates the need for proper handling and processing of seafood especially marine crabs. It is also important that at household level proper hygienic measures should be taken to avoid cross contamination.

Keywords : Vibrio parahaemolyticus Marine Crabs Biofilm Food Borne

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