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Development and Evaluation of Chicken Meat Nuggets Enriched with Date Palm Fruit (Phoenix dactylifera L.)

Dipanwita Bhattacharya Gurunathan Kandeepan Pranav Chauhan
Vol 8(7), 287-295
DOI- http://dx.doi.org/10.5455/ijlr.20170316074748

A study was conducted to enrich the chicken nuggets with date palm fruit as it is a good natural antioxidant as well as sensory property enhancer. Dried date palm fruits were soaked in boiling water to prepare date palm paste and it was incorporated into chicken emulsion replacing 2.5%, 5% and 7.5% chicken meat to prepare chicken nuggets. A control group of chicken nuggets was made for experiment. Emulsions were stuffed into stainless steel boxes to steam cook for 45 minutes to prepare chicken nuggets. Proximate composition, total phenolics, TBARS, total plate count etc were conducted as quality parameters of chicken nuggets using date palm. 7.5% date palm incorporated chicken nuggets showed less lipid oxidation as TBARS value was lowest 0.20±0.004 and highest total phenolic content of 0.19±0.01. But 5% date palm incorporated chicken nuggets showed high acceptability in sensory evaluation. Up to 5% level of date palm added chicken nuggets were highly acceptable though 7.5% showed high in phenolic content, higher sweetness and less in lipid oxidation.


Keywords : Antioxidant Date Palm Nuggets Phenolic Content

Fruit and vegetable extracts incorporation in processed meat products provide broad-spectrum health benefits. There is a growing tendency among meat eaters to assimilate sufficient vitamin, mineral, dietary fibre, and phenolic components in meat products. Antioxidant effect of fruits comes mainly because of its polyphenolic compounds in it. Fruits and vegetables used in processed meat industry exhibit functional properties such as water binding, fat emulsification, improving yield and sensory properties (Serdaroglu et al., 2005). Processed food products contain so many valuable components, such as dietary fibers, pigments, sugars, organic acids, flavors and antibacterial substances (Lario et al., 2004; Ku and Mun, 2008).

Incorporation of antioxidants in the diet has beneficial effects on human health because they protect biologically important cellular components, such as DNA, proteins, and membrane lipids, from reactive oxygen species (ROS) attacks. Fruits in general, are a good of natural source which may protect us against chronic diseases by reducing oxidative damage to biomolecules (Al-Shahib and Marshall 2003; Fu et al., 2011). The date palm (Phoenix dactylifera Linnaeus) is an important member of the family Arecaceae (Palmae), providing an essential food for millions of people in the arid and semi-arid regions of the world. Egypt, Iran, Saudi Arabia, South East Spain are gifted with enormous production of dates. In India, commercial cultivation of date is raising day by day. The major date producing states are Rajasthan, Gujrat, Tamil Nadu and Kerala. Though our consumption is very less compared to other countries, we stand in the first position of importing huge quantity of about 4 lakhs tonnes of dates every year. Hababouk, kimri, khalal, besser, rutab and tamar are various ripening stages of dates and during these stages important changes take place in chemical composition including decrease in phenolic substances with increase in sugar concentration (Baliga et al., 2011). Some important health benefits of date are constipation reliever and bowel movement regulator, prevents iron deficiency anaemia, reduces heart disease risk, prevents colon cancer, and traditional medicine as immune system stimulator (Puri et al., 2000; Martín-Sánchez et al., 2013). Extension of processed meat products with date fruits could reduce production costs and improve the nutritional qualities of the products.

Therefore, a study was conducted by incorporating different levels of date palm purée in meat emulsion. This particular study aims to evaluate the effect of date palm purée on physiochemical, microbiological and sensory properties of chicken nuggets prepared from emulsion.

Materials and Methods

Raw Materials

Dressed chicken carcasses were procured from Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India. Carcasses were packed in clean low density polyethylene (LDPE) bags and quickly brought to the processing plant of Livestock Products Technology Division, IVRI, Bareilly. After proper de-boning, meat was packed in polyethylene bags and stored overnight at 4 ± 1 °C in a refrigerator and then frozen stored at −18 ˚C till further use. Dried date palms (Tamr stage) were bought from local market of Bareilly. They were soaked (ratio 1:2, palm: boiling water) for 3 min to inactivate fruit enzymes and, after a cooling-down, they were pitted, peeled and blended in a mixer to have the date palm purée.

Preparation of Chicken Nuggets

Four different meat batters were prepared at different times which included replacement of lean chicken meat with increasing concentration of date palm purée. 2.5%, 5% and 7.5% freshly prepared date palm purée were incorporated in chicken nuggets of T1, T2 and T3 respectively.  A control group of nuggets were also prepared following same procedure but without substituting date palm in the formulation. In the processing of chicken nuggets, de-boned lean chicken was minced first then salt, sodium tri-polyphosphate, and sodium nitrite were dissolved in chilled water, then added to minced meat and mixed for 3 minutes at medium speed in bowl chopper. This was followed by addition of oil and chopping for 3 min. condiments, refined wheat flour, dry spices and finally date palm purée were added separately to the mixture which was again mixed for 1 min each for each ingredient. The mixture was removed and stuffed in stainless steel boxes with parchment paper smeared with oil inside and steam cooked for 45 min. Chicken meat blocks so obtained were cooled, sliced and cut into cuboidal pieces to get nuggets.

Table 1: Formulation of ingredients for the preparation of control and treatment chicken nuggets (g/100g)

Ingredients (gkg-1) Control T-1 T-2 T-3
Lean meat 70 67.5 65 62.5
Date palm 2.5 5 7.5
Refined oil 9.5 9.5 9.5 9.5
Chilled water 10 10 10 10
Condiments 3.4 3.4 3.4 3.4
Salt 1.8 1.8 1.8 1.8
Maida 3.2 3.2 3.2 3.2
Spice mix 1.5 1.5 1.5 1.5
STPP 0.5 0.5 0.5 0.5
Sodium nitrite 0.15 0.15 0.15 0.15

STPP: sodium tripolyphosphate; T1-T3: chicken nuggets, where chicken meat was substituted by 2.5%, 5% and 7.5% date palm purée

Product Analysis

Proximate Composition and Microbiological Quality Analysis

Proximate compositions of all the samples were performed on the day following manufacturing of product. Proximate composition of the product such as percentage moisture, fat, protein and ash was determined as per AOAC (1997). The pH was determined by the standard method as outlined by Trout et al. (1992) using combined glass electrode of Elico pH meter (Model; LI 127, India). Water activity was measured at 25 °C in a water activity meter (Novasina, TH-500, Axair Ltd., Pfaeffikon, Switzerland). Standard plate counts (SPC) of samples were done on the day of preparation of product as per standardized methods (APHA, 2001).

 

Cooking Yield

Cooking yield (%) was calculated and expressed as percentage by the following formula. Percent cooking yield was determined by calculating weight differences for samples before and after cooking (Murphy et al., 1975).

TBARS

2-Thiobarbituric acid reactive substances (TBARS) were determined on freshly prepared samples (zero day) according to Tarladgis et al. (1962). Sample absorbencies were measured with spectrophotometer (Model: Beckman DU 640, USA) at 538nm. Reagents were obtained from Sigma (UK). A conversion factor of 7.8 was used multiplying the OD in the calculation of TBARS. Each sample was analysed in duplicate and results were expressed as 2-thiobarbituric acid reactive substances (TBARS) as mg malonaldehyde (MDA)/kg sample.

Total Phenolics

Total phenolic content in cooked chicken nuggets from control and date purée incorporated formulations was analysed by using the Folin-Ciocalteu assay (Escarpa and Gonzalez, 2001) with slight modifications. Briefly, five grams of nugget were homogenised with 25 ml of 70% acetone and kept overnight for extraction at refrigeration temperature. Suitable aliquots of extracts were taken in a test tube, and the volume was made to 0.5 ml with distilled water followed by the addition of 0.25 ml Folin-Ciocalteu (1 N) reagent and 1.25 ml sodium carbonate solution (20%). The tubes were vortex mixed and the absorbency was recorded at 725 nm after 40 min.

Sensory Evaluation

Sensory evaluation of various samples were conducted using an 8 point descriptive scale (Keeton, 1983), where 8 = excellent and 1 = extremely poor. The sensory panel involved ten members selected from scientists and post graduate students of the division of Livestock Products Technology with age between 28–45 years. The panellists were explained about the nature of experiments without disclosing the identity of samples and were asked to rate their preference on 8 point descriptive scale. Samples were warmed in a microwave oven for 1 min. Water was provided to rinse mouth between the samples. The panellists judged the samples for general appearance, binding, colour, flavour, juiciness, and overall acceptability. Sensory evaluation was done in sensory evaluation laboratory of livestock products technology division of Indian Veterinary Research Institute. Each panellist evaluated three replicates of all the samples at room temperature (Macfie, 1989).

Statistical Analysis

Three trails were conducted for each experiment with duplicate samples. The data generated for different quality characteristics were compiled and analysed using SPSS (version 20 for Windows; SPSS, Chicago, USA) with variance and least significant difference. Statically analysed results were tabulated and the results were interpreted as in tables.

Result and Discussion

pH

The pH value between control and treatments differed significantly (p<0.05) in Table 2. The pH values decreased significantly (p<0.05) with increasing concentration of date palm in chicken nuggets.

Table 2: Physico-chemical and microbiological parameters of date palm purée incorporated chicken nuggets

Parameters Control T1 T2 T3
pH 6.44±0.003a 6.19±0.003b 6.17±0.003c 6.17±0.002c
Water Activity 0.98±0.000a 0.97±0.000a 0.97±0.002a 0.96±0.00a
Moisture (%) 65.08±0.004d 65.15±0.009c 65.36±0.21b 66.12±0.02a
Crude Protein (%) 18.33±0.005a 18.28±0.007b 18.07±0.01c 17.69±0.01d
Fat (%) 8.62±0.008a 8.60±0.006a 8.24±0.017b 7.76±0.016c
Ash (%) 2.14±0.009d 2.30±0.01c 2.60±0.008b 2.83±0.02a
Shear Force value (Kg/cm2) 0.45±0.006a 0.40±0.005b 0.37±0.004c 0.31±0.007d
Cooking Yield (%) 91.57±0.55c 94.33±0.18b 93.56±0.54b 95.50±0.07a
TBARS (mg malonaldehyde/Kg) 0.29±0.007a 0.26±0.004b 0.25±0.008b 0.20±0.004c
Total Phenolics (mg/GAE 100g) 0.03±.004d 0.08±0.01c 0.16±0.01b 0.19±0.01a
SPC (log cfu/g) 3.24±0.02a 3.05±0.03b 2.90±0.02c 2.68±0.02d

T1-T3: chicken nuggets where chicken meat was substituted by 2.5%, 5% and 7.5% date palm purée;  Means with different superscripts (letters row wise) indicates statistical significance P ˂ 0.05.

But T2 and T3 did not differ significantly (p<0.05). Date palm incorporated chicken nuggets showed lower pH than control which may be due to presence of organic acids in it. It was reported that due to date palm purée addition there was a tendency of lowering in pH for uncooked low fat beef patties (Yıldız-Turp and Serdaroglu, 2010). In another study it was found that chicken nuggets showed a decreased pH due to apple pulp incorporation (Verma et al., 2010). It was reported that date paste contains so many organic acids which lower its pH (Sánchez-Zapata et al., 2011). In another study it was found that pH decreased with enhanced concentration of date paste in pork liver-pate (Martín-Sánchez et al., 2013). Similarly, it was noticed that the pH also decreased for pea incorporation in chicken nuggets (Polizer et al., 2015; AOAC, 1997).

Water Activity

There was no significant (p<0.05) difference in water activity between control and treatment groups as found in Table 2. Minimal changes were noticed in treatment groups. In treatment groups, reduced values were noticed for water activity (aw). The changes may be due to similar water activity values of date palm and control chicken nuggets. Some workers also did not find any appreciable changes in water activity in control and date paste incorporated chicken nuggets (Martín-Sánchez et al., 2013).

Proximate Composition

Slight variations were detected in proximate composition between the control and treatment chicken nuggets in Table 2. Moisture content and ash content alleviated significantly (p<0.05) in date purée incorporated chicken nuggets. 7.5% date paste added nuggets showed maximum moisture content. Similarly, protein content and fat content decreased significantly (p<0.05) in treatment groups. Groups with higher moisture content showed the least protein and fat content in treatment groups. A study reported that moisture content was 66.41±0.54 g/100g in date paste (Martín-Sánchez et al., 2013) and in another study a very similar result was obtained in the proximate composition of cooked meat product with date paste (Sánchez-Zapata et al., 2011). Similar study was reported in English breakfast sausages added with different amount of functional ingredients viz. albedo, oat fibre, wheat flour rusk and its combinations (Aleson-Carbonell et al., 2005). A similar result was also obtained in chicken nuggets treated with pea fibre (Polizer et al., 2015).

Shear Force Value and Cooking Yield

Shear force value decreased significantly (p<0.05) in treatment groups in Table 2. The values decreased slightly between date purée treated groups. Increased tenderness in treatments might be due to presence of organic acids in date fruits and its higher moisture content. A significant (p<0.05) difference was found in cooking yield parameter between control and treatments. Between T1 and T2 there was no significant (p<0.05) difference. T3 showed the highest cooking yield percentage. Increase in cooking yield might be due to water retention properties of fibres in date purée, it was reported that incorporation of fruit fibre and cereals in dry fermented sausage resulted in an increase in cooking yield (Garcia et al., 2002). Similarly, incorporation of fruits and vegetables also increased in cooking yield of meat products (Mehta et al., 2013).

TBARS

Addition of date palm purée significantly (p<0.05) reduced the TBARS value in treatments compared to control sample in Table 2. The 2.5% and 5% concentration of date paste did not show significant differences whereas, the 7.5% concentration indicated the highest reduction in TBARS value. The result indicated that active ingredients present in date purée decreased the lipid oxidation which could affect product quality, sensory attributes and nutritional aspects of meat product. It was reported that date palm fruit contains poly-phenolic compounds and flavonoids as antioxidants (Tarladgis et al., 1960). The date palm paste showed reduced TBARS values but they did not differ significantly with control in few studies (Martín-Sánchez et al., 2013). Similar result was obtained when date plum powder incorporated sliced hams showed decreased TBARS values (Nunez et al., 2008).

Total Phenol Content and SPC

Total phenolic content increased significantly (p<0.05) with increasing concentration of date palm as shown in Table 2. In the microbiological study, standard plate count decreased significantly (p<0.05) in treatment groups when compared to control as in Table 2. It was earlier reported that total phenolic content ranged from 149 to 836 mg of GAE/100 g fresh weight (Nuñez et al., 2009) and some workers also found that date palm paste contains 225±22 mg GAE/100 g fresh weight total phenol content (Sánchez-Zapata et al., 2011). Further, it was also found that date palm fruit showed antibacterial activity for both gram positive and gram negative bacteria (Mansouri et al., 2005; Saleh and Otaibi, 2013).

Sensory Evaluation

Significant differences (p<0.05) were noticed between control and treatment groups for all sensory attributes listed in Table 3.

Table 3: Sensory analysis scores of date palm purée incorporated chicken nuggets

Parameters Control T1 T2 T3
Appearance 7.17±0.05c 7.37±0.03b 7.70±0.04a 7.43±0.02b
Colour 7.4±0.03b 7.68±0.03a 7.75±0.06a 7.28±0.03c
Flavour 7.08±0.03c 7.25±0.02b 7.6±0.03a 6.98±0.03d
Juiciness 7.10±0.02c 7.25±0.01b 7.54±0.02a 7.10±0.05c
Binding 7.07±0.00d 7.23±0.01c 7.30±0.00b 7.36±0.00a
Overall acceptability 7.09±0.00d 7.26±0.00b 7.51±0.03a 7.17±0.03c

T1-T3: chicken nuggets where chicken meats were substituted by 2.5%, 5% and 7.5% date palm purée; Means with different superscripts (letters row wise) indicates statistical significance P ˂ 0.05.

Among them, 5% date palm paste incorporated chicken nuggets got high preference for acceptability. There was no significant (p<0.05) difference in colour between T1 and T2. In case of flavour, T3 showed the least score but for binding it scored highest. Date palm is sweet fruit and sweetness comes from the soluble solids present in it (Sánchez-Zapata et al., 2011) whereas, date fibre might be responsible for high binding. Juiciness was highest in T2 that may be due to increased moisture content. Except flavour, all the sensory parameters showed improved scores in date palm treated meat products compared to control. Some workers also reported that date purée incorporation enhanced sensory scores in pork liver paste (Martín-Sánchez et al., 2013).

Conclusion

Date palm purée can be incorporated into chicken nuggets up to a maximum level of 5% without affecting meat product quality attributes. Incorporation at 7.5% level showed increased phenolic content and cooking yield but decreased lipid oxidation, SPC, besides contributing to improved sweetness in the meat product which was viewed as negative factor by the sensory panellists.

Conflict of Interest

We certify that there is no conflict of interest with any financial organisation regarding the material discussed in the manuscript.

Acknowledgement

Authors are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, for providing the necessary facility for conducting this research work.

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