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Diagnosis of Mycobacterium bovis Using Interferon-gamma (IFN-γ) Assay and Post Mortem in Indian Cattle (Bos indicus)

Sanjeev Kumar Shukla Shubhra Shukla Anuj Chauhan Sarvjeet Kumar Rajveer Maurya Sudesh Rathaor Lakshya Veer Singh Manjit Panigrahi
Vol 7(9), 148-158

Bovine tuberculosis (bTB) is a major economic problem in animal husbandry. The aim of this study, we evaluated the capability of the interferon-gamma (IFN-γ) assay to identify cattle infected with Mycobacterium bovis. A total of one hundred sixty nine randomly selected animals, one year and above were studied for the prevalence of bovine TB using Single Intradermal Comparative Cervical Tuberculin (SICCT) Test, Bovine Gamma-Interferon (γ-IFN), Polymerase Chain Reactions (PCRs) and Post-mortem based examination. The collected blood samples for IFN testing simultaneous with the SICCT, as well as seven and twenty one day later. All selected cattle samples were processed polymerase chain reaction and got 248 bp amplification. In the diagnostic tool the sensitivity of IFN was 94.7%, while the specificity was 89.9%. After injection of PPD applied seven or twenty one days, the sensitivity was 76.6 and 74.7%, respectively, whereas the specificity was 89.9 and 83.3%. Post mortem examination was done by the experts of M. bovis infected twenty five animals and we got eight positive. Detection of cattle tuberculosis can be done in early stage in live animals with most realistic approach like IFN-γ assay, PCR and post-mortem examination to accelerate the eradication process, therefore, more studies should be performed to further improve them.

Keywords : Cattle Interferon Gamma Assay Mycobacterium bovis Tuberculin Skin Test

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