This study was undertaken to know the effects of dietary Monosodium Glutamate (MSG) on growth performances of broiler chickens. Three hundred twenty day-old Ross 308 chicks were randomly divided to 5 treatment groups of 64 chicks each. Negative and positive control groups were fed on basal diet without supplement (R0-) and 1g of antibiotic (R0+) respectively and the 3 others groups were fed on diets supplemented with 1 mg, 2 mg and 4 mg of MSG/kg of feed. Results revealed that feeding broilers with MSG decreased (p<0.05) FI at the starter phase with an upward trend at the finisher phase. Diet supplemented with 2 mg of MSG/kg increased (p<0.05) LBW and WG, and decreased FCR. MSG significantly (p<0.05) increased lactic acid bacteria counts as compared to E. coli and Salmonella. Hematological parameters and histology of organs were not affected while serum content in protein, globulin, triglyceride, total cholesterol and urea markedly increase. It was concluded that 2 mg of MSG/kg could be used as feed additive to improve growth performance and mitigate the public concern about bacteria resistance issues as well as antibiotics residues in broiler chickens.