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Effect of Dietary Supplementation of Synthetic L-Lysine and DL-Methionine on Growth Performance, Nutrient Utilization and Immunity in Commercial Broiler Chicken

Bornalee Handique Gunaram Saikia Runjun Dowarah Bibeka Nanda Saikia Joga Dev Mahanta
Vol 9(3), 225-232

An experiment was conducted to investigate the effect of synthetic L- lysine and DL-methionine on growth performance, nutrient utilization and humoral immunity in commercial broilers. 144 Day old broiler chicks (Vencobb 400) were randomly divided into four groups (3 replicates of 12 each) and basal diet supplemented with synthetic L-lysine @ 0, 20, 30 and 40% and DL-methionine @ 0, 30, 40 and 50% higher than BIS (2007) recommendation for 42 days. Supplementation of synthetic L-lysine and DL-methionine improved (P<0.001) final body weight and gain in weight at finisher phase as compared to control. The best feed conversion ratio (FCR) and protein efficiency ratio (PER) was observed in T2 group supplemented with 20% lysine and 30% methionine. Higher (P<0.05) digestibility of crude protein and fibre and nitrogen retention was observed by supplementation of L-lysine and DL-methionine in broiler chicken. Supplementation of synthetic L- lysine and DL-methionine improved humoral immune response in the experimental birds with higher antibody titre.

Keywords : Broiler DL-Methionine Growth Performance Immunity L-Lysine Nutrient Utilization

Due to increase in world population there is a serious problem in providing food for all people. Poultry meat is the source of animal protein for fight against malnutrition and to overcome the problem of food scarcity. Therefore, in recent year’s poultry farming is the profitable and fastest growing food industry in animal husbandry sector. Preparation of balanced poultry feeds requires various feed ingredients, among all the feed ingredients protein rich feed ingredients cost higher. Cereal grains are deficient in critical amino acids like lysine, methionine, threonine and tryptophan (Smith, 2001). However, the animal protein supplements such as fish and meat meal though rich in these essential amino acids but their use in poultry ration are not affordable for their short supply, very expensive and restriction for use due to adulteration.  Quality of the protein is more important than the quantity of protein used. The major problem facing poultry farmers is high cost of protein feed resources but it can be overcome by  supply the right quantity and quality of protein i.e. amino acids in the diets. Since, amino acids are responsible for protein synthesis in the body which is the primary constituents of structural and productive tissues such as skin, feathers, bone matrix, ligaments, soft tissues and different organs. Lysine is essential for protein synthesis, regulation of cell division, calcium absorption, mobilization of fatty acid into the mitochondria and helps in enhancing overall growth performance, breast meat yield, efficiency of feed utilization, carcass protein retention and reduces fat deposition. Methionine plays roles in poultry such as involve in protein synthesis, acts as methyl donor for cellular metabolism and formation of coenzyme S-adenosylmethionine involves in polyamine synthesis, act as sulphur donor and as lipotropic agent. Considering the above facts present experiment was conducted to study the effect of dietary supplementation of synthetic L-lysine and DL-methionine in excess of BIS (2007) recommendation for broiler chicken.

Materials and Methods

This experiment was conducted in the experimental poultry shed, Department of Animal Nutrition, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, Assam . A total of 144 day old broiler chicks (Vencobb strain 400) randomly distributed into 4 treatment groups (3 replicates of 12 chicks each) using randomized block design viz., T0-basal diet (control), T1, T2 and T3 were offered the basal diet supplemented with synthetic L- lysine @20, 30 and 40 percent and DL- methionine at 30, 40 and 50 percent in excess of the total BIS (2007) requirement. The basal diet was prepared as per BIS (2007) without supplementation of L-lysine and DL-methionine (Table 1). All the chicks were reared in deep litter system under similar environmental conditions (temperature 20-27oC and relative humidity 78-88%) and had access to ab libitum feed and water during the entire experimental period. The chicks were vaccinated for Ranikhet disease with Lasota strain (F1) vaccine on 7th day and 14th day of age Infectious Bursal Disease (IBD) vaccine (dose as per company’s recommendation). Performance of the broiler in respect of weekly feed intake, digestibility of nutrients, weekly body weight and body weight gain, feed conversion ratio (FCR), protein efficiency ratio (PER) were calculated.



Table 1:  Percent ingredient and nutrient composition of the experimental basal diet

Attributes Starter (Kg) Finisher (Kg)
Percent Ingredient Composition
Maize 51 54.3
De-oiled rice polish 5.5 7.2
De-oiled GNC 15 10
Soybean meal 23.5 22.5
Vegetable oil 3 4
Mineral mixture 1.5 1.5
Salt 0.5 0.5
Nutrient Composition on DM basis (%)
Dry matter 92.23 92.09
Crude protein 22.08 20.01
Crude fibre 5.78 5.1
Ether extract 4.2 4.3
NFE 54.79 57.59
Total  ash 13.15 13
*ME(Kcal/kg) 3130 3202
*Lysine 1.2 1
*Methionine 0.5 0.45

*Calculated values (N.B. Vitamin premix (Vitablend vit A, B2, D3, K) was added @ 20 g per quintal of diet in both starter and finisher diet.  Mineral mixture contained calcium 25%, Phosphorus 5%, Sodium chloride 23%, Iodine 10 ppm, Copper 100 ppm, Manganese 2000 ppm and Cobalt 10 ppm.

At the end of the feeding trial (after 42 days), a metabolism trial was conducted for five days (2 days adaptation and 3 days of collection) to assess the digestibility and balance of nutrients. Humoral immune response was also measured at the end of the feeding trial. The data’s were analyzed as a randomized complete block design using GLM procedures of SPSS (version 17.0 for Windows; SPSS, Chicago, III., U.S.A). Difference among treatment means were determined by one way and two-way ANOVA and compared according to Duncan’s multiple range test (Duncan, 1995).

Results and Discussion

Growth Performance

The change of body weights in supplemented groups (T1, T2 and T3) were significantly higher (P<0.001) as compared to control group (T0) during 4-6 weeks of feeding (Table 2). Whereas, non-significant (P>0.05) effect had been observed in average body weight change among the experimental groups during 0-3 weeks of age. Similarly, gain in weight (g/bird) from 0-3 weeks was also did not show any significant effect due to supplementation of synthetic L- lysine and DL-methionine in the diet (Table 2). The present findings were accordance with the report of Kalbande et al. (2009) observed in broilers fed on different level of synthetic methionine supplemented diets. Batal and Dale (2006) reported that the benefit of increase lysine levels may not be seen at early stage of growth but at the end of the finisher phase. Higher (P<0.001) total gain in weight was observed in supplemented groups (T1, T2 and T3) as compared to control (T0) whereas, T2 and T3 groups had better (P<0.001) total gain (0-6 weeks) among the treatment groups.  It might be due to the effect of balance of both lysine and methionine and their combined effect in improving feed and nutrient utilization and more availability of lysine and methionine for protein synthesis. This corroborates with the finding of Bowa et al. (2008) reported that consumption of lysine and methionine resulted in significant increase in weight gain. Feed intake in the broilers did not showed any significant difference among the experimental groups during the entire experimental period. Contrary to the present findings, Pillai et al. (2006) reported higher (P<0.05) feed intake with addition of high level of methionine in the broiler ration. However, there was a slight increase of feed intake at high level of lysine and methionine supplemented groups that might be the improvement of the quality of feed due to amino acid balance. The observed values of the total feed consumption by the experimental birds were comparable to the values reported by Kalbande et al. (2009), Ahmed and Abbas (2011 and 2015) and Zhai et al. (2016).

Table 2: Effect of synthetic L-lysine and DL-methionine on growth performance in commercial broiler chicken

Attributes Dietary Treatment SEM P value
T0 T1 T2 T3
Average Body Weight
0 day 42.89±0.57 42.89± 0.55 43.33±0.50 42.17±0.49 0.26 0.464
0-3 week 644.80±4.20 646.80±13.20 647.70±11.9 668.80±12.70 12.7 0.53
4-6 week 1754a±23.90 1843b±26.80 1963b±26.90 1925b±23.60 14.2 <0.001
Gain in Weight (g/bird)
0-3 week 306.50±9.42 308.70±9.52 321.00±9.52 332.80±8.66 4.68 0.166
4-6 week 424.20a±8.00 414.10a±9.28 501.30c±9.50 458.60b±9.15 5.29 <0.001
Total gain 1713.2a±23.9 1799.9b±26.8 1923.4c±27.0 1882.6c±23.5 14.2 <0.001
Feed Intake (g/bird)
0-3 week 857.5±29.4 857.1±8.07 900.1±28.9 981.4±31.4 23.9 0.052
4-6 week 2433.5±17.4 2407.3±5.58 2453.0±8.58 2426.9±17.5 9.44 0.145
0-6 week 3291±21.0 3214±25.1 3353±37.8 3408±14.1 73.17 0.881
0-3 week 1.55±0.12 1.39±0.17 1.41±0.22 1.39±0.06 0.08 0.424
4-6 week 2.18b±0.01 2.01b±0.01 1.86a±0.01 1.92b±0.01 0.05 <0.001
0-6 week 1.92b±0.05 1.79b±0.03 1.75a±0.01 1.81b±0.11 0.13 0.042
0-3 week 3.69±1.01 3.99±1.29 3.69±0.30 2.52±0.21 0.36 0.76
4-6 week 2.18a±0.01 2.34b±0.15 2.48c±0.01 2.36b±0.10 0.05 <0.001
0-6 week 2.93±0.37 3.16±0.36 3.08±0.11 2.94±0.24 0.12 0.925

*abcMean values with different superscripts within row differ significantly

The feed conversion ratio (FCR) was narrowest in 0-3 weeks of age in the all four experimental groups but wider in 4-6 weeks of age (Table 2). T2 group showed the best (P<0.05) FCR as compared to other groups during 4-6 weeks and 0-6 weeks of the experimental period. It might be due to better effect of lysine and methionine supplementation in nutrient utilization resulting highest body weight gain of broilers in this group where effective balance of lysine and methionine in the diet. Similar pattern of findings were reported by Osti and Pandey (2004). Numerically similar value of FCR in broilers were reported by Kalbande et al. (2009), Kanduri et al. (2013), Ahmed and Abbas (2015) by  feeding diet supplemented with synthetic and herbal amino acids.

Protein efficiency ratio (PER) during the whole experimental period in all the treatments were decreased along with the increase of age (Table 2). Significant differences (P<0.001) were found in 4-6 weeks of age; where supplemental groups (T1, T2.and T3) showed significantly better (P<0.001) PER than control group. Present findings were comparable to the experiment reported by Ahmed and Abbas (2011) where broiler diets were supplemented with methionine at higher level (120 and 130%) than NRC recommendation. The better PER in T2 group might be due to better nutrient utilization of crude protein digestibility and higher nitrogen retention. This result was in agreement with the findings of Onu et al. (2010) where supplementation of synthetic lysine and methionine give better result as compare to un-supplemented group.

Nutrients Digestibility and Balance

Apparent dry matter and CP digestibility was better (P<0.05) in supplemented groups compared to control (Table 3).

Table 3: Effect of synthetic L-lysine and DL-methionine on digestibility and balance nutrient in broiler chicken

Attributes Dietary Treatment SEM P value
T0 T1 T2 T3
Nutrient Digestibility
DM 64.74a±1.09 76.97b±0.85 77.90b±2.43 75.43b±0.98 1.05 0.004
CP 61.16a±1.77 64.99b±1.67 71.79c±1.07 63.06b±3.16 1.64 0.003
CF 21.95±0.28 21.97±0.20 24.73±0.11 22.11±0.10 0.1 0.899
EE 64.76±4.19 72.64±5.11 73.75±3.16 68.69±5.68 2.79 0.06
NFE 53.36±1.77 52.05±1.35 54.03±0.94 51.97±0.81 0.605 0.672
Nutrient Balance
Nitrogen 60.93a±93 64.99b±0.68 71.78c±0.69 63.06b±0.56 0.51 0.01
Calcium 51.19±0.89 51.93±0.55 51.37±0.84 51.38±0.94 0.37 0.925
Phosphorus 50.38±1.89 50.64±1.06 50.41±0.43 50.33±1.86 0.65 0.999

abcMeans with different superscripts within the same row differ significantly.

It might be due to better feed utilization by the bird due to adequate balance of amino acid in the diet.  T2 group had better (P<0.05) digestibility amongst the supplemental groups. Non-significant (P>0.05) differences were observed between the experimental groups in respect of EE, CF and NFE digestibility in the commercial broilers (Table 2). The present study was in agreement of finding of Onu et al. (2010). Higher (P>0.05) CF digestibility in T2 was due to increase efficiency of utilization of energy and protein providing optimum environment for bacterial growth in the large intestine. Similar digestibility co-efficient of CF in broiler were reported by Sonowal (2008). There was significant (P<0.05) difference in percent nitrogen retention among the experimental groups and the values were higher (P<0.05) in T2 group as compared to other groups. It might be due to digestive environment creating by adequate balance of amino acid. Present findings were similar with findings of Gorman and Balnave, 1995; Si et al., 2001 and Xu et al., 2003. The retention of both calcium and phosphorus were found to be non-significant (P>0.05) among the experimental groups for entire experimental period (Table 3). Similar finding was reported by Ogunwole et al., 2017.

Humoral Immune Response

The humoral immunity (HI) was expressed as the antibody response to sheep erythrocytes (SRBC) deploying HA test. Antibody titre value (log2 titre) was maximum on 5th day of post inoculation (Table 4).

Table 4: Effect of synthetic L-lysine and DL-methionine on antibody titre (log2 titre) against sheep RBC in broilers chicken

     Treatment Period   Significant
3d 5d Mean ±SE T P TXP
T0 0.90±0.14 1.05±0.15 0.98a±0.17 0.014






T1 1.50±0.28 1.35±0.15 1.43b±0.22
T2 1.20±0.20 1.80±0.00 1.50b±0.36
T3 1.20±0.00 1.20±0.00 1.20ab±0.16
Mean ±SE 1.20±0.09 1.35±0.11  

*abMeans in the same column bearing different superscripts differ significantly

The supplemented groups had showed higher (P=0.014) antibody titre in comparison to control (T0), however T1 and T2 groups had higher (P=0.014) immune response than T3 group. It might be due to lysine and methionine constructively affects the humoral immune response. Similar findings were reported by Shini et al. (2005) and Bouyeh (2012) by diet supplemented with lysine and Methionine (as TSAA) more than NRC (1994) recommendation.


Dietary supplementation of synthetic L-lysine and DL-methionine @ 30 and 40% respectively influenced the growth performance, utilization of dry matter and crude protein, balance of nitrogen and humoral immune status in broiler chicken. This clearly suggests that supplementation of L-lysine and DL-methionine could be strategy to increase performance and immunity in broilers. However, further studies were required to ascertain the observed findings in the present study.



The authors express thankfulness to the Dean, Faculty of Veterinary Science, AAU, Khanapara for providing necessary facilities to carry out the experiment.


  1. Ahmed, M. E. and Abbas, T. E. 2011. Effects of dietary levels of methionine on broiler performance and carcass characteristics. International Journal of Poultry Science, 10(2): 147-151.
  2. Ahmed, M. E. and Abbas, T. E. 2015. The effect of feeding herbal methionine versus DL-methionine supplemented diets on broiler performance and carcass characteristics. International Conference on Agricultural, Ecological and Medical Sciences. Paper presented at Penang (Malaysia), 10-11.
  3. Batal, A. B. and Dale, N. M. 2006. Lysine: sources, bioavailability and factors affecting requirement. MS Thesis, University Georgia, Athens, GA, USA.
  4. 2007. Bureau of Indian Standard: Poultry Feed Specification. 4th Review, Manak Bhawan, Bahadur Shah Zafar Marg, New Delhi, India.
  5. Bouyeh, M. 2012. Effect of Excess Lysine and Methionine on Immune system and Performance of Broilers. Annals of Biological Research, 3(7):3218-3224.
  6. Bowa, G. S.; Tegbe, T. S. B.; Ogendipe, S.O. and Dafang, I. I. 2008. Effect of amino acid supplementation of labbab seed diet on performance of young pigs. Proceeding of the 33rd annual Conference of Nigeria Society for Animal Production, 350-380.
  7. Coon, C. N. 2002. Feeding egg type replacement pullets. In Commercial Chicken Meat and Egg Production. (Bell, D.D. and Weaver, W.D. Eds.), pp. 267-285. Cluwer Academic Publishers, Dordrecht, Netherland.
  8. Duncan, D. B. 1995. Multiple range and multiple F-test. Biometrics II, 1-42.
  9. Gorman, I. and Belnave, D. 1995. The effect of dietary lysine and methionine concentrations on growth characteristics and breast meat yield of Australian broiler chicken. Australian Journal of Agricultural Science, 46(8): 1569-1577.
  10. Kalbande, V. H.; Ravikanth, K.; Maini, S. and Rekhe, D. S. 2009. Methionine Supplementation Options in Poultry. International Journal of Poultry Science, 8(6): 588-591.
  11. Kanduri, A. B.; Saxena, M. J.; Ravikanth, K.,;Maini, S.; Dandale, M. and Kokane, S. S. 2013. Performance Assessment of Broiler Poultry Birds Fed on Herbal and Synthetic Amino Acids. Advances in Bioresearch, 4(3): 26-28.
  12. Ogunwole, O. A.; Babatunde, O. O.; Faboyede, R. A.; Adedeji, B. S. and Jemiseye, F. O. 2017. Calcium and phosphorus retention by broiler chickens fed groundnut cake based-diet supplemented with l-lysine and dl-methionine. Animal Production Research Advances, 29(1):240-248.
  13. P. N.; Ayo-Enwerm, M. C. and Ahaotu, E. O. 2010. Evaluation of carcass characteristics and cost effectiveness of broiler chick’s fat synthetic lysine and methionine supplemented soya bean based diet. Journal of Nutrition, 1(11): 22-26.
  14. Osti, N. P. and Pandey, S. B. 2004. Methionine and Lysine Supplementation in Low Quality Feed Ingredient based diets on the performance of broiler chicken. Journal of Poultry Science, 1-9.
  15. Pillai, P. B.; Fanatico, A. C.; Beers, K. W.; Blair, M. E. and Emmert, J. L. 2006. Homocysteine remethylation in young broilers fed varying levels of methionine, choline and betaine. Poultry Science, 85: 90-95.
  16. Shini, S. Li.; Mulyantini, X.; Zhang, N. G. A. D.; Shini, A., Kumar, A.; Hosking, B. J. and Bryden, W. L. 2005. Methionine requirements and immune function in broiler chicks. Recent Advances in Animal Nutrition in Australia, 15: 13A.
  17. Si, J.; Fritts, C.A.; Burnhan, D. J. and Waldroup, P. W. 2001. Relationship of dietary lysine level to the concentration of all essential amino acids in broiler diets. Science, 80: 1472-1479.
  18. Smith, A.J. 2001. Poultry. The Tropical Agriculturalist series (CTA). Rev. Edn., Macimillan Education Ltd., Oxford. London.
  19. Sonowal, M. 2008. Growth performance and nutrient utilization of broiler chicken at different dietary levels of treated and untreated mustard oil cake. Ph.D. Thesis, Assam Agricultural University, Khanapara, Guwahati-22.
  20. Xu, Z. R.; Wang, M. Q.; Mao, H. X.; Zhan, X. A. and Hu, C. H. 2003. Effect of L- carnitine on growth performance, carcass composition and metabolism of lipid in male broilers. Poultry Science, 82: 402-413.
  21. Zhai, W., Peebles, E. D., Schilling, M. W. and Mercier, Y. 2016. Effects of dietary lysine and methionine supplementation on Ross 708 male broilers from 21 to 42 d of age (I): growth performance, meat yield, and cost effectiveness. Journal of Applied Poultry Research, 25:197–211.
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