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Effect of Lysozymes, Antibiotics and Probiotics on Growth Performance and Biochemical Parameters in Broiler Chickens

Surajit Baksi Pravinsinh Chauhan Nirav Rao Ashish Chauhan

The objective of present study was to determine the comparative effect of lysozymes with enrofloxacin and Lactobacillus as growth promoters in broiler chickens. Two hundred-day old broilers were selected and randomly divided into four treatment groups. Each group had five repeats with 10 birds in each replication. Treatments with lysozyme, enrofloxacin and Lactobacillus improved the growth performance, but feed conversion ratio was poor. Body weight and feed conversion ratio were increased by 2.94% and 2.91%, respectively in lysozyme supplemented group as compared to control group. It was more or less similar with enrofloxacin and Lactobacillus supplemented group. Biochemical parameters (activity of lysozyme, total protein, blood glucose, cholesterol, AST and GPT) of birds were also measured and it was observed that lysozymes improved the health performance and immune functions of broiler chickens and can be used as an alternative to growth promoters like antibiotics and probiotics. Antibiotic, Broiler Chickens, Lysozyme, Probiotics

Keywords : Antibiotic Broiler Chickens Lysozyme Probiotics

How to cite: Baksi, S., Chauhan, P., Rao, N., & Chauhan, A. (2019). Effect of Lysozymes, Antibiotics and Probiotics on Growth Performance and Biochemical Parameters in Broiler Chickens. International Journal of Livestock Research, 9(10), 74-78. doi: 10.5455/ijlr.20190626100638


Use of antibiotic as growth promoters in poultry feed for several years led to the development of antibiotic resistant pathogens (Abdul et al., 2019; Dar et al., 2012; Gordon and Taylor, 1954; Gong et al., 2002). Lysozymes can be a potential alternative (1, 4-β-N-acetylmuramidase). It cleaves the glycosidic bond between the N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan which is an important component of cell walls (Grossowicz and Ariel, 1983), thus it provides protection against bacterial infection. Lysozyme with an alternative to antibiotics for poultry is relatively limited. Zhang et al. (2006) conducted an in vitro experiment to find an effective dose of lysozyme for controlling Clostridium perfringens and they found that 200 μg/ml dose completely inhibited the growth of Clostridium perfringens and thereby inhibited production of α-toxin which caused the lesions associated with necrotic enteritis (NE). A follow up study by Zhang et al. (2010) evaluated a radiant energy-treated lysozyme antimicrobial blend to control NE in broiler chickens. This blend at a level of 200 ppm was as effective as commonly used antibiotics (bacitracin methylene salicylate (BMD)) for treatment of NE in the broiler chickens. Liu et al. (2010) found that 40 ppm of exogenous lysozyme effectively reduced the number of Clostridium perfringens in the ileum of broilers and prevented intestinal lesions. The aim of present study was to evaluate comparative effect of lysozyme with antibiotic and probiotic on the growth performance and biochemical parameters of broiler chickens (Jin et al., 1998).

Materials and Methods

Trials were conducted using four groups. Two hundred-day old broilers were selected and randomly divided in four treatment groups. Each group had five repeats and 10 birds in each replication. Diets were isocaloric and isonitrogenous within the starter (days 0-14) and finisher (days 15-42). The diets were fed in mash form throughout the trial. The four treatment groups were: control (no growth promoters), lysozyme (350-400 mg/ton), antibiotic (enrofloxacin-10 mg/kg body weight) and probiotic (Lactobacillus culture) groups. Diets were formulated to meet or nutrient requirements and analysed as shown in Table 1 (AOAC, 2005).

Table 1: Ration composition of broiler starter and finisher ration

Ingredients Starter (g) Finisher (g)
Corn 558.2 586.6
Soyabean meal 371.7 341.5
Oil (veg) 27 34
Dicalcium Phosphate 19.2 17.2
Limestone 12.2 11
Salt (NaCL) 4.5 4.5
Choline chloride 2.6 2
DL- Methionine 2.2 1.1
Mineral premix 2 2
Vitamin Premix 0.2 0.2
L- Lysine HCL 0.3 0
Total 1000.1 1000.1

In this experiment, birds were mass weighed per pen on days 0, 21, and 42. The feed remaining in the feeders was weighed on each day. The daily consumption and feed conversion ratio (FCR) was calculated. At the end of the trial, blood samples from 10 birds in each group were collected for biochemical analysis. Biochemical parameter like total protein, total cholesterol, blood glucose, aspartate transaminase (AST) and glutamatepyruvate transaminase (GPT) were estimated (AOAC, 2005). All data were statistically analysed using IBM SPSS 20 software (version 20.0).

Results and Discussion

Growth Performance

The growth performance of each group is presented in Table 2.

Table 2: Effect of various treatments on growth performance of broiler chickens

Items Control Lysozyme group Antibiotic group Probiotic group
Initial body weight (0th day) 44.63 ± 0.50 43.74 ± 0.81 43.81 ± 0.23 44.19 ± 0.31
21 days body weight 472.97 ± 3.90 521.61 ± 5.16a 482.51 ± 3.28a 497.86 ± 1.41a
42 days body weight 2140.96 ± 6.59 2203.22 ± 1.87b 2156.55 ± 9.97 2188.52 ± 4.94b
FCR 2.06 ± 0.13 2.12 ± 0.11c 2.08 ± 0.09 2.11 ± 0.13c

Data are significantly different from control group (p < 0.05); Data are significantly different from Control and antibiotic group (p < 0.05); Data are significantly different from Control and antibiotic group (p < 0.05)

There was significant improvement in body weight of treated groups as compared to control. Improved performance in feed conversion ratio was also observed in treated groups. The body weight and FCR were increased by 2.94% and 2.91%, respectively in lysozyme supplemented group as compared to control group. It was more or less similar with probiotic supplemented group. In antibiotic treated group, there was increase in body weight with no significant difference in FCR as compared to control. Similar observations were found by Abdel-Latif et al., 2017 and Torki et al., 2018, where growth performance and intestinal micro biota were improved in birds fed with lysozymes.

Biochemical Parameters

The biochemical parameters were found as shown in Table 3.

Table 3: Biochemical parameters of various treatment groups

Group Name Biochemical Parameters
Lysozyme (µmol/L) Total Protein (g/L) Blood glucose (mg/dL) Total Cholesterol (µmol/L) AST(μ/L) GPT(μ/L)
Control 2.84± 0.11 19.66± 0.76 177.99± 1.22 3.49± 0.12 177.32± 1.91 24.08± 0.31
Lysozyme group 3.66± 0.05a 27.56± 1.7a 176.27± 0.71 2.64± 0.05a 184.22± 0.79a 24.36± 0.85
Antibiotic group 2.72± 0.16b 20.85± 1.29b 174.67± 1.25 3.46± 0.05b 181.22± 3.63 16.3± 0.41ab
Probiotic group 2.96± 0.09b 23.65± 0.38ab 177.21± 1.49 3.37± 0.08b 184.59± 0.79a 17.42± 0.07ab

Significant as compared to Control group (p<0.05) for individual parameters; Significant as compared to Lysozyme treated group (p<0.05) for individual parameters

Presence of lysozyme activity was found more in lysozyme treated group as compared to control group (28.73%). It was observed in present study that the amount of protein was found significantly (p < 0.05) high in lysozyme treated group as compared to others (40.18%, 32.18% and 16.53% more as compared to control, antibiotic and probiotic group, respectively). There was no significant influence of different treatments on blood glucose level. Serum cholesterol level was found to be significantly (p < 0.05) lower in lysozyme treated group as compared to control which suggests that lysozymes are effective in reducing fat deposition in broiler birds. Liver function parameters like AST and GPT were also measured in birds and there was no influence of different treatments on AST., although GPT levels in antibiotic and probiotic treated groups were found to be less as compared to control, which is indicative of liver function abnormality. Similar findings were observed by Rahman et al., 2013, where probiotics and enzymes treatment improved haematological parameters of broilers.


Supplementation of lysozymes in broiler ration could improve the growth performance (Gong et al., 2017). It also improves blood biochemical indices which leads to better health performance and immune functions of broiler chickens. Hence, lysozyme may be used as an alternative to growth promoters, like antibiotics which will avoid drug resistance in the human being who eats the broiler meat.


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