Dairy cattle assigned to four treatment groups were administered PGF2α analogue (Cloprostenol) on day 8 (PG8) (n=4), day 25 postpartum (n=5), or antibiotic from day 1 to 5 postpartum (n=5), or kept as untreated control (n=4). The monitoring of uterine involution of previous gravid and non-gravid uterine horn was performed by transrectal ultrasound scanning at weekly interval and significant difference (P<0.05) was found between PG8 and control group. Body condition score (BCS) recorded at the time of calving and there was significant difference (P<0.05) in all treatment groups. Endometrial cytology confirmed sub-clinical endometritis in cows under control group based on the mean percentage of PMN cells. However, PMN cells were not statistically different (P>0.05) in all the groups.