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Effect of Supplementation of Butylated Hydroxytoluene on Post-Thaw Viability, Motility and Membrane Integrity of Crossbred Bovine Spermatozoa

Anoop Singh Mridula Sharma Shiv Prasad Yaqoob Bhat Anil Kumar Dinesh Pandey Santosh Shukla
Vol 7(4), 93-101
DOI- http://dx.doi.org/10.5455/ijlr.20170324025755

This study was conducted to evaluate effect of butylated hydroxytoluene (BHT) on post thaw semen quality of cattle bull. Thirty six ejaculates from two bulls were collected and extended with glycerolated egg yolk tris diluter. Samples were divided into three groups according to BHT concentration as 0 mM (C) 0.5 mM (T1) and 1.0 mM (T2). The samples were analyzed for progressive motility, sperm viability and membrane integrity at three stages i.e. post dilution (stage I), post equilibration (stage II) and post thaw (stage III). Progressive motility, sperm viability and sperm membrane integrity were significantly (p<0.05) higher in T1 & T2 compared to the control. The concentration 1mM of BHT showed better results as compared to 0.5 mM. BHT addition improved the sperm quality probably due to its antioxidant property as it reduces the lipid peroxidation of the sperms.

Keywords : Sperm Viability Butylated hydroxytoluene Membrane Integrity Antioxidant Crossbred Bovine Spermatozoa

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