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Evaluation of Anthelmintic Activity (In -vitro) of Boerhaavia diffusa Linn (Punarnava) Root Extract Including Phytochemical Screening

Shelu R. S. Jangde C. R. Nanotkar R. Y. Limsay R. P. Awasthy V. B. Singh R. P.
Vol 7(5), 209-219

The plant Boerhaavia diffusa is a herb found throughout the India. The roots of the plant have been described in various herbal natural medicine literatures to possess many medicinal properties like anti-inflammatory, antibacterial, diuretic, antiurethritis, hepatoprotective, anti-convulscent, antinematodal and antifibrinolytic etc. In view of various medicinal uses, the root of the plant was selected for screening of anthelmintic property. The helminths including Monezia expansa, Haemonchous contortus and Amphistomes were used for study. The B. diffusa alcoholic extract at 10, 20, 30 mg/ml concentration can produced paralysis and death of worm within half to five hrs of exposure. Plant root extract at higher dose (30mg/ml) reported significant anthelmintic activity by causing paralysis and death, when compared to standard drug against three of heminths. The extractability percentage with absolute alcohol was worked out to be 8%. The phytochemical screening reveals the presence of sterol, alkaloid, amino acid, protein, reducing sugar, glycoside, saponins, tannins, and flavonoides. This study provides scientific evidence that the root of B diffusa has anthelmintic property.

Keywords : Anthelmitic Boerhaavia diffusa Linn Helminths Paralysis

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