The study was conducted to explore polymorphism in bovine growth hormone gene in 210 indigenous cattle of North East India (viz. Manipur, Meghalaya, Mizoram, Nagaland, Assam and Tripura) and crossbred cattle. Two different variants of bGH gene viz. A and T were detected from DNA using PCR-RFLP. The frequency of A allele was predominant (0.667) among indigenous cattle. On the contrary, the frequency of T allele was predominant (0.867) in crossbred cattle. Among the genotypes, AA genotype was found in intermediate to high frequency among the local cattle of North East India. While the genotype AA was completely absent in the crossbred population. The population conforming to equilibrium indicated lack of selection pressure in these cattle population.