Histochemical localization of oxidative and hydrolytic enzymes was conducted in bursa of Fabricius of four different age groups of broiler chicken viz., day-old, two, four and six weeks. Epithelium covering the bursal plicae showed moderate positive reaction and bursal follicles showed mild positive reaction to alkaline phosphatase (ALP) and acid phosphatase (ACP). However, interfollicular connective tissue, Tunica serosa and Tunica muscularis showed negative reaction to ALP and ACP activity. Tunica muscularis, cytoplasm of cortical and medullary lymphocytes, epithelium lining the plicae and in the cortico-medullary junction and star-shaped cells in the medulla of some follicles showed positive reaction for all dehydrogenases. Strong α-naphthyl acetate esterase activity was observed in the epithelium covering the bursal plica. Increased cytochrome oxidase activity was observed as age advanced.