E. Raja Ravi Teja N. Rajendranath Vol 7(12), 111-113 DOI- http://dx.doi.org/10.5455/ijlr.20170717041511
In this modified alizarin red-s method the fetuses were fixed in 10 per cent Neutral buffered formalin for 1 week. Reduced the potassium hydroxide digestion time to secure the muscular system and then stained with alizarin red-s. Excess stain was removed by fresh 4 per cent potassium hydroxide solution. Stained fetuses were cleared for 2 weeks by using two clearing agents. The primary ossification centers of the skull bones were clearly visible. The fetuses were preserved in glycerin.
Keywords : Alizarin Red-S Fetus Primary Ossification Centers Potassium Hydroxide Skull