Isolation, Identification and Antimicrobial Susceptibility Profile of Salmonella spp. from Poultry Farms in Jos South Local Government Area of Plateau State, Nigeria
M. Y. Sugun J. Dalis S. Mailafiya M. Odugbo S. Isa G. A. Hashimu
Vol 9(6), 69-78
The study was carried out to biochemically determine the presence of salmonella spp., in poultry farms, detect the presence of 16S DNA using conventional polymerase chain reaction and to know the antimicrobial susceptibility profile of the isolates circulating in poultry farms in Jos south local government area of Plateau state, Nigeria. Grams staining culture and biochemical property identified 9(39%) as Salmonella spp. where 7(2.1%) were identified as Salmonella gallianarum by being non-motile and 2(0.6%) were the other motile Salmonellae and 5(6.0%) samples generated amplification products of 284 bp after the PCR with specific primers which targeted invA gene associated with Salmonella spp. Most of the isolates were resistant to the drugs tested. Five of the isolates were susceptible to Ciprofloxacin, two isolates were susceptible to Chloramphenicol and Neomycin and only one isolate was susceptible to Sulphamethoxazole/Trimethoprim. This study highlighted the occurrence of resistance to important antimicrobials like amoxicillin and erythromycin. As antimicrobials are easily obtainable over the counter, there is need for veterinary authorities in the state to regulate antimicrobial use in poultry farms and in clinics to limit the spread of multidrug-resistant salmonella among poultry farms.
Keywords : Antimicrobial Susceptibility Isolation Molecular Characterization Salmonella spp.
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