Current study was carried out in four veterinary divisions of Kilinochchi district to study adoption of artificial insemination for cattle and the factors influencing artificial insemination in the Kilinochchi district of Sri Lanka. Out of the 640 cattle farmers 265 farmers were selected using table of random numbers. Information was collected using structured questionnaire. Data were processed using Microsoft Excel 2007 and statistical analysis was performed with Proc Freq, Chi-Square and Glm in SAS. The results revealed that salary/wages were the primary income of cattle farmers in the Kilinochchi district. The main reason for keeping cattle was milk and manure. The cattle breeds mainly found in the study area were indigenous (49%) and Jersey crosses (46%). Farmers adopting semi intensive system of management ranged from 87-97%. Overall more than 93% of the famers used Jersey crosses to inseminate their animals. Framers adopting both natural and AI were around 41%. Major reasons for adoption of AI were unavailability of good bull in herd in all divisions (22%), advantageous (easy to do) (17%) and to get healthy calves (15%). The main reasons for non-adoption of AI technology in the Kilinochchi were difficulties in heat detection, cost and distance of AI center from the farm. Credit facilities and grazing field were the major constraints for cattle farming in the Kilinochchi district.