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Molecular Characterization and Phylogeny of Wolbachia endosymbiont of Dirofilaria immitis from NE States of India

Sonjoy Kumar Borthakur Parimal Roy Choudhury Gautam Patra Subhamoy Ghosh
Vol 8(5), 189-195
DOI- http://dx.doi.org/10.5455/ijlr.20171108100954

The role of obligate endosymbiont Wolbachia in the pathogenesis and immune response to dog heartworm disease is well documented. In the present study, we explored some of the molecular characterization and phylogenetic study of endosymbiont-Wolbachia of canine heartworm (Dirofilaria immitis) from Northeastern state of India. Understanding the role of this endosymbiont as specific biomarker for presence of canine heartworm, a molecular characterization and study the phylogenetic relationship among other isolates from different parts of the world is carried out using FtsZ gene. The nucleotide sequence was submitted to GenBank and accession number obtained (JX524742). The percent identity among the Wolbachia endosymbiont in D.immitis ranges from 99.4 to 99.8 per cent. Phylogenetic characterization showed close identity with Wolbachia endosymbiont of USA isolate.

Keywords : Dirofilaria immitis India Northeastern States Wolbachia

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