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Molecular Detection and Therapeutic Management of Malasseziosis in Cattle

P. Ameldev P.V. Tresamol Aarsha Raj Mery Rincy
Vol 8(4), 154-160

Yeast of genus Malassezia inhabit the skin of variety of mammals and birds and are considered as opportunistic pathogens in animals and man. Malassezia dermatitis among three cows from an eco-farm in Thrissur Kerala state, with skin lesions on the dorsal aspect neck and their successful treatment is described. Upon physical examination hyperkeratosis along with circular crusty lesions were noticed. Examination of skin scraping with 10% potassium hydroxide was found to be negative for fungal spores/mites. Hair along with the skin scrapings were cultured in sabouraud’s dextrose agar with chloramphenicol at 37ºC for four weeks and resulted in greyish white, raised convex type colony after 12 hours of incubation. Grams staining of the isolates revealed presence of dark blue coloured budding yeast cells suggestive of Malassezia. No other fungal organisms were isolated during a period of four weeks. DNA was extracted from the isolates by using DNeasy blood and tissue kit (Qiagen) and subjected to a genus specific PCR using primers 5’ AGCGGAGGAAAAGAAACT3’ and 5’GCGCGAAGGTGTCCGAAG. Gel electrophoresis of the PCR products revealed 600bp product suggestive of Malassezia genus. The restriction -endonuclease analysis of PCR products confirmed the species as Malassezia restricta. The case was successfully treated.

Keywords : Cattle Dermatitis Malasseziosis Molecular Diagnosis

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