Polymorphisms in Exon-1 and Partial Intron-1 of Growth Hormone Gene and its Association with Body Weight Traits in Broiler
Sunita Meena G. C. Gahlot U. Pannu Durga Devi Hemlata Chouhan M. Ashraf Hiteshwar Singh Yadav
Vol 9(4), 114-119
In this study chicken growth hormone (cGH) gene polymorphism has been investigated in broiler by PCR-RFLP. The genomic DNA was extracted from 150 samples by using modified spin column technique. The DNA fragment of the growth hormone gene with 776 bp was amplified by PCR using specific primers. Then the PCR products were digested with MspI restriction enzyme and analyzed on 8% polyacrylamide gel. The allelic frequency of exon-1 and partial intron-1 locus for A and B allele were 0.39 and 0.61, respectively. The genotypic frequency for AA, AB and BB in Broilers were 0.22, 0.28 and 0.50, respectively. The result showed that the exon-1 and partial intron-1 of cGH is polymorphic.
Keywords : Chicken Growth Hormone Polymorphism PCR-RFLP
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