Twenty faecal samples of buffaloes in an individually labeled polythene bags were collected every month from eight different region of Malwa viz. Indore, Dhar, Jhabua, Dewas, Ujjain, Ratlam, Shajapur and Agar from July 2018 to February 2019 i.e. total 1280 faecal samples. Overall prevalence of gastrointestinal parasites was 35.63%. The highly prevalent gastro-intestinal parasites were strongyle (12.27%) followed in order by Eimeria spp. (7.58%), amphistomes (6.95%), Fasciola spp. (5.31%), Toxocara spp. (4.45%), Moniezia spp. (2.27%), Trichuris spp. (1.48%) and Strongyloides spp. (0.78%). Mean egg per gram ± S.E. of strongyles (205±9.83), Strongyloieds spp. (150±23.57), Toxocara spp (155.93±9.21), Trichuris spp. (190±22.21) amphistomes (230.10±19.61), Fasciola spp. (226.47±16.84), Moniezia spp. (134.48±9.14) and Eimeria spp. (291.78±25.36) was recorded. Different genera of strongyle species found in buffaloes in descending order of their prevalence were Haemonchus spp. (82.54%), Trichostrongylus spp. (8.36%), Oesophagostomum spp. (4.26%), Nematodirus spp. (2.31%), Cooperia spp. (1.43%) and Ostertagia spp. (1.10%). The EPG/OPG in most of the fecal samples was in the range of 100-300 and indicated moderate infection that warrants treatment.