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Screening of Enrofloxacin and Its Metabolite Ciprofloxacin Residues by High-Performance Liquid Chromatography in Cow Milk of District Udham Singh Nagar, Uttarakhand

M. Srinivasu Abul Hasan Ahmad Nirbhay Kumar Disha Pant Wasif Ahmad
Vol 7(7), 140-145
DOI- http://dx.doi.org/10.5455/ijlr.20170520035753

Residues in animal products play significant role in development of resistance to antibiotics. A plenty of resistance cases has been demonstrated in farm conditions making the veterinary practice vulnerable. To monitor the levels of enrofloxacin and its metabolite ciprofloxacin residues in the raw milk samples of cow, 100 samples collected from different blocks of U.S.Nagar were analysed for enrofloxacin and ciprofloxacin residues and positive samples were detected and quantified using High Performance Liquid Chromatography (HPLC). Out of 100 milk samples analysed 19 samples (19%) had detectable amount of residue levels for enrofloxacin and ciprofloxacin and 2 samples (10.52%) had residue levels above the recommended Maximum Residue Limit MRL. A linear calibration curve was obtained by plotting area against concentration with a correlation coefficient of 0.9975 while average recoveries were greater than 86.8% with relative standard deviation (RSD) value of 0.32%.The method described in the present study would be helpful for monitoring of enrofloxacin and ciprofloxacin residues in milk.

Keywords : Residues Enrofloxacin Ciprofloxacin Milk Samples HPLC

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