NAAS Score 2019


Declaration Format

Please download DeclarationForm and submit along with manuscript.


Free counters!

Previous Next

Serological Detection of Mycobacterium bovis from Suspected Cattle by Fluorescence Polarization Assay

Rabyia Javed Deepti Narang Mudit Chandra S. T. Singh G. Filia
Vol 9(11), 114-121

A Fluorescence polarization assay (FPA) was optimized and validated to detect antibodies to Mycobacterium bovis in cattle sera. A total 192 (cattle and buffalo) blood samples were screened for the CMI response by Comparative Intradermal (CID) tests and Gamma interferon (IFN-γ) tests as per the standard protocol of OIE, World Organization of Animal Health 2009. The presence of antibodies is indicative of prior infection with Mycobacterium bovis. Out of 192 animals screened, 14 and 23 animals were positive by CID and IFN-γ respectively. The diagnostic test revealed the results as out of 33 samples ( positive samples by either of the two tests) and subjected to FPA, 7 samples were positive by confirmatory protocol given by the kit (milli-polarisation >50). A total of 53 animals (33 of which were positive by either of the CID and IFN tests, while 20 were negative by either of the CID and IFN-γ tests) were subsequently selected for the FPA. The FPA detected 12/53 (22.64%).

Keywords : Antibody CID FPA

Full Text Read : 18 Downloads : 9
Previous Next

Submit Case Reports for Special Issue (Dec’19)

Recommend IJLR to include in UGC-CARE list

Download Completed format here

IJLR_UGC CARE Recommendation


Recommendations of new journals should be routed by universities and colleges as follows:

  1. Universities: IQAC cell to respective regional CARE University
  2. Affiliated colleges: College IQAC cell to parent university’s IQAC cell. Parent university IQAC cell will forward to respective regional CARE University.

You can find Zonal UGC-CARE address here