NAAS Score 2020

                   5.36

UserOnline

Free counters!

Previous Next

Seroprevalence of Newcastle Disease Virus in Guineafowl in Live Bird Market Sokoto, Nigeria

B. R. Alkali B. I. Mashi M. D. Usman A. B. Shuaibu A. H. Jibril
Vol 8(5), 83-88
DOI- http://dx.doi.org/10.5455/ijlr.20180102034811

This study used serological method to estimate the level of circulating antibodies against Newcastle disease (ND) in apparently healthy guinea fowl, raised under traditional management system obtained from the major live bird market of Sokoto State, Nigeria. Competitive Enzyme Linked Immunosorbent Assay was used to analyze two hundred and twenty nine (229) sera for Newcastle disease virus antibodies from randomly selected guinea fowls presented at live bird market. Overall, seroprevalence of 40.6 % (93/229) was detected. The male specific prevalence was 41.9 % (83/198) while female specific prevalence was 32.3 % (10/31) detected. Age specific prevalence showed adults guinea fowl to had 44.4 % prevalence while younger ones had 29. 1%. The findings of this study as it’s relate to epidemiology and transmission dynamics of ND is discussed.


Keywords : Guinea Fowl Live Bird Market Newcastle Diseases Sokoto State Seroprevalence

Introduction

Newcastle disease is a major viral disease of economic importance in poultry and rated as one of the greatest constraints to the development of rural poultry production in Nigeria and in most developing countries, causing serious threats to poultry industry (Anosa and Adene, 2007; Oladele et al., 2003). The disease had been reported to be acute, rapidly spreading, contagious, nervous and respiratory disease of birds of all ages (Okeke and Lamorde, 1988). The clinical signs of ND are known to vary based on the virulence and tropism of the ND virus involved, species, age, immune status of the birds, as well as, the prevailing environmental condition (Alders and Sprabrow, 2002). ND is endemic often causing outbreak in backyard and commercial poultry in most parts of Africa and Nigeria (Ezeokoli et al., 1984Adene, 1996). The impact of ND was said to be more in Nigeria where over 90% of the poultry were rural poultry that were left to roam freely to scavenge for food and water bringing them into close activity space with wild birds (Adene and Oguntade, 2006).

It is pathogenic for the domestic chickens, turkeys, guinea fowls and other poultry. Wild birds may not show clinical disease but do develop antibodies to the virus (Orajaka et al., 1999; Sa’idu et al., 2004). These birds act to maintain ND viruses which serve as sources of frequent outbreaks in rural and backyard poultry (Alexander, 2001Sa’idu et al., 2004). Outbreak of ND had been caused by contamination of poultry feed with faeces of pigeon in the UK (Alexander, 2011). Wild birds and semi-domestic birds are known to be susceptible and develop antibodies to ND hence, play major roles in the spread of ND viruses especially in Nigeria as well as other African countries with poor poultry husbandry practice (Oladele et al., 1996Sa’idu et al., 2004). The circulation of ND viruses in wild birds and maintenance in the environment were responsible for most outbreaks in backyard and commercial poultry even after vaccination (Sa’idu et al., 2008). Natural outbreak of Newcastle disease (ND) was reported in a flock of guinea-fowl in Nigeria, affecting 1,029 birds of which 250 (24.3%) died. Paralysis of the legs and wings, coughing, sneezing, white diarrhoea and complete cessation of egg production were observed (Haruna et al., 1993).

Live bird market has been identified as one of the high risk areas for disease transmission due to high concentration and interaction of a wide variety of birds brought from different sources (Jibril et al., 2014). In view of this, this study was conducted to determine the prevalence of Newcastle Disease Virus among apparently healthy guinea fowls in live bird market of Sokoto state in order to determine the seroprevalence of the disease among guinea fowl that were presented in the market.

Materials and Methods

Study Area

The study was conducted in Sokoto. Sokoto is the capital of Sokoto State, located at 13°N and longitude 30°E and 90°E in the North Western part of Nigeria. It lies roughly between longitude 30°E and 15°E of Greenwich and between 4°N and 14°N of the equator. It covers approximately an area of 56,000 square kilometers (Anon, 2001). Sokoto metropolis is located in the Sudan savannah zone with grass vegetation, sandy soil and humidity, which is usually below 40% except in few wet months when it approaches 60% (Iloeje, 1971).

Sample Size Determination

Sample size was estimated using the formula of Thrusfield, 2005.

n = z2 p (1-p)/d2

Where: n = number of samples, z = standard normal deviation at 95 % confidence interval, p = expected prevalence (p = 17.0 %, Abraham et al., 2014), d = desired absolute precision and q = 1-p, n = 217.

A total of 229 samples were collected to increase the chance of antibody detection.

Sample Collection

Data forms were used to record vital information of each bird sampled before sample collection. A total of 229 blood samples were collected from randomly selected guinea fowls at the point of slaughter.  Samples were transported in an ice park at 4°C container to the City Campus Central laboratory, Usmanu Danfodiyo University Sokoto, Nigeria, where the sera were harvested. Clotted blood samples were centrifuged (BOSCH ® Digital Centrifuge USA) at 3000 revolutions per minute for 5 minutes. The clear sera were then harvested by decantation and delivered into corresponding clean plain vials that were appropriately labeled. All sera were stored at -20ºC (VT-8, Denmark) to preserve their immunological and other biochemical properties prior to serology (Brown and Torres, 2008).

Serology and Serological Techniques

Sera samples were screened for the presence of antibodies to Newcastle Disease Virus using ID Screen® NDV competition obtained from ID-vet Innovative Diagnostics, Montpellier, France. The test is a competitive ELISA for the detection of Nucleo-Protein antibodies in serum of poultry. The principle of this technique is based on the formation of a colourless reaction following interaction of a positive test serum in a well, coated with NDV antigen after addition of a chromogenic substrate. Optical density was read using ELISA reader (Optic System IVYMEN® 2100C, USA) at 450nm.

Data Analysis

The data obtained was analysed using GraphPad Instat ®. Chi-square was used to measure the strength of association between sex, age and prevalence of NDV. Values of P < 0.05 at 95 % confidence interval were considered significant.

Result

The overall prevalence was found to be 40.6 % (93/229). Table 1, indicated the sex specific prevalence of Newcastle Disease virus in guinea fowl. The males showed a higher prevalence rate (41.9: 83/198). However, this difference is not statistical significant (p > 0.05). In Table 2, adult guinea fowls shows a higher prevalence rate when compared with the young birds.

Table 1: Sex specific prevalence of Newcastle Disease Virus in guinea fowl in live bird Market of Sokoto, Nigeria

Sex Number Sampled Number Positive Prevalence (%) Relative Risk 95 % CI
Male 198 83 41.9 1.299 0.7603-2.229
Female 31 10 32.3 1
Total 229 93 40.6

χ2 = 0.675; p value = 0.41; CI = confidence interval

Table 2: Age specific prevalence of Newcastle Disease Virus in guinea fowl in live bird Market of Sokoto, Nigeria

Age Number Sampled Number Positive Prevalence (%) Relative Risk 95 % CI
Young 24 7 29.1 0.6571 0.345-1.249
Adult 205 91 44.4 1.00  
Total 229 93 40.6    

χ2 = 1.460; p value = 0.227; CI = confidence interval

Discussion

This serological study revealed the presence of circulating antibodies of Newcastle disease (ND) among apparently healthy guinea fowls in Sokoto major live bird market, with 40.6% of guinea fowls tested positive. This shows that ND is still an endemic viral disease of domestic poultry in the study area (Saidu et al., 2004). Antibodies detected most likely would be as a result of natural infection since vaccination of the village poultry is rarely undertaken in Nigeria (Abdu et al., 1987). Guinea fowl, turkeys and peacocks are susceptible to ND and local husbandry practices, where different species of birds are raised together in the same compound, encourage cross infection by ND virus among the different species (Saidu et al., 2004). The detection of high prevalence of circulating antibodies to ND in the live bird market is a major concern in relation to other birds. These birds are exposed to birds from multiple sources having a higher tendency of circulating the virus and may serve as a source of infection to house hold chickens when introduced (Killian, 2009; Jibril et al., 2014). This study however, shows a relatively lower prevalent rate of ND virus in village chickens when compared with what has been reported earlier (41%) by Adu et al., (1986). Ezeokoli et al.(1984) reported 73% prevalence of antibodies against NDV in traditionally managed backyard flocks in Zaria while Orakaja et al. (1999) reported 63% in south eastern Nigeria. El-Yuguda et al. (2007) reported a prevalence of 46% in village chickens in Borno State; Salihu et al. (2012) reported an ND prevalence of 54.67% in Nasarawa State. This observed differences in the rates of NDV antibodies may be as a result of ecological variations in ND activity or sampling methods and may perhaps be a reflection of the impact of environment on the viability of NDV and epidemiology (Orajaka et al., 1999). However, studies conducted by Ameji et al. (2011) and Jibril et al. (2014) has showed a lower prevalence of 25.5% and 35.8 % in live bird markets of Kogi and Zamfara State respectively in Nigeria. Studies in other part of Africa have reported a similar prevalence in unvaccinated local chickens. This study shows a higher prevalence in male (41.9 %) when compared to females (32.3 %) guinea hen. This report is similar to the studies conducted by Jibril et al. (2014) that showed a higher prevalence among males’ chickens in live bird markets and housed hold in Zamfara State, Nigeria. However, recent studies conducted by Alkali et al. (2017) have showed a higher prevalence rate among females (15.32 %) than males (14.02 %).

From this study, adult guinea fowls show a higher prevalence (44.4 %) of antibodies to NDV when compared with younger ones (29.1 %). However, studies conducted by Alkali et al. (2017) has showed that, higher prevalence was recorded in young birds (Less than three months) and least in adults (13.66%).

Conclusion

Newcastle disease in endemic in the study area, live bird markets is an important factor to be considered in the transmission dynamics of Newcastle disease virus. The disease is presence in all sexes and ages of guinea fowl in the study area.

References

  1. Abdu, P. A., S. U. Abdullahi, A. A. Adesiyun, and C. D. Ezeokoli, “Challenge study on infectious bursal disease in chicks derived from vaccinated hens,” Tropical Animal Health and Production, Vol. 19, no. 1, pp. 47–52, 1987.
  2. Abraham-Oyiguh J, Sulaiman LK, Meseko CA, Ismail S, Suleiman I, Ahmed SJ,
    Onate EC. Prevalence of Newcastle disease antibodies in local chicken in federal capital territory, Abuja, Nigeria. International Scholarly Research Notices. 2014;30.
  3. Adene, D.F. and A.E. Oguntade, 2006. The structure and importance of the commercial and rural based poultry industry in Nigeria. Nigerian Poultry Sector Report, FAO. Rome, pp: 1-70. http://www.fao.org/docs/eims/upload//214281/ReviewNigeria.
  4. Adene, D.F., 1996. International poultry health problems: Perspective from the poultry industry in Africa. Proceedings of the 20th World Poultry Congress, September 1-5, 1996, New Delhi, India, pp: 401-414.
  5. Adu, F.D., Edo, U. and Sokale, B. (1986). Newcastle disease: The immunological status of Nigerian local chickens. Tropical Veterinarian, 4: 149-152.
  6. Alders, R and P. B. Spradbrow, Controlling Newcastle Disease in Village Chickens, Monograph Australian Center for International Resources, Canberra, Australia, 2002.
  7. Alkali, B. R., A. B. Shuaibu, I. Bello and M. D. Usman (2017) Seroprevalence of Newcastle Disease Virus in Local Chickens from Sokoto, Nigeria, Microbiology Research Journal International 19(1): 1-5, 2017; Article no.MRJI.31362.
  8. Ameji, O. N., P. A. Abdu, and L. Saidu, “Seroprevalence of Avian influenza, Newcastle disease and Gumboro disease in chicken in Kogi State, Nigeria,” Bulletin of Animal Health and Production African Journal, vol. 59, no. 4, 2011.

9.    Anon (2001): Sokoto State-the seat of the Caliphate. In: Sokoto State Government Dairy.

10.              Anosa, G. N. and D. F. Adene, “Comparative tissue reactivity of lentogenic strains of Newcastle disease vaccines in Nigeria,” Nigerian Veterinary Journal, vol. 28, no. 3, pp. 6–10, 2007. S. B. Oladele, P. Abdu, K. A. N. Esievo, A. J. Nok, and N. M. Useh, “Prevalence of Newcastle disease virus antibodies in chickens reared in Zaria,” in Proceedings of the 28th Annual Conference of Nigerian Society of Animal Production, vol. 28, pp. 7–9, 2003.

  1. Brown C, Torres A. USAHA. Foreign Animal Diseases, seventh Edition. Committee of Foreign and Emerging Diseases of the US Animal Health Association Boca Publication Group; 2008.
  2. EL-Yuguda, A.D., Ngulde, I. S., Abubakar, M. B. and Baba, S. S. (2007). Village chicken health, management and production indices in selected villages of Borno State, Nigeria: Family Poultry Journal, 17(1, 2):112-132.
  3. Ezeokoli, C.D., J.U. Umoh, A.A. Adesiyun and P.A. Abdu, 1984. Prevalence of Newcastle disease virus antibodies in local and exotic chickens under different management systems in Nigeria. Bull. Anim. Health Prod. Afr., 32: 253-257.

14.              Haruna ESShamaki DEcheonwu GOMajiyagbe KAShuaibu YDu DR.(1993) A natural outbreak of Newcastle disease in guinea-fowl (Numida meleagris galeata) in Nigeria. Rev Sci Tech. 1993 Sep; 12(3):887-93.

15.              Iloeje, N.P. (1971). Where is Nigeria and who are the Nigerians? In: A new Geography of Nigeria, Vol. 1, pp 14.

16.              Jibril, A. H., J. U. Umoh, J. Kabir, L. Saidu, A. A. Magaji, M. B. Bello, and A. A. Raji (2014) Newcastle Disease in Local Chickens of Live Bird  Markets and Households in Zamfara State, Nigeria. ISRN Epidemiology. Vol. 2014, article ID 513961, 4 pages.

17.              Killian, M. L. “National veterinary services laboratories avian influenza and Newcastle disease diagnostics report,” in Proceedings of the 113th Annual Meeting of the United States Animal Health Association, pp. 590–593, 2009.

  1. Okeke, E. N. and A. G. Lamorde, “Newcastle disease and its control in Nigeria,” in Viral Disease of Animals in Africa, A. Olufemi Williams and W. N. Masiga, Eds., CTA/OAU/STRC/Publication, Lagos, Nigeria, 1988.
  2. Orajaka, L.J.E., D.F. Adene, B.M. Anene and E.A. Onuoha, 1999. Seroprevalence of Newcastle disease in local chickens from Southeast derived Savannah zone of Nigeria. Revue d’Elevage Medecine Veterinaire Pays Tropicaux, 52: 185-188.
  3. Sa’idu, L., L.B. Tekdek and P.A. Abdu, 2004. Prevalence of Newcastle disease antibodies in domestic and semi-domestic birds in Zaria, Nigeria. Veterinarski Arhiv, 74: 309-317.
  4. Salihu, A.E, Chukwuedo, A.A, Echeonwu, G.O.N., Ibu, J.O., Chukwuekezie, J. O., Ndako, J., Junaid, S.A., Onovoh, E.M., Paul- Abu, L.G., Ujah, A. E., Dalyop, A.K., Tende, M.D., Shittu, I., Chindo, H. Z. and Umahi, N.F. (2012). Seroprevalence of Newcastle disease virus infection in rural household birds in Lafia, Akwanga and Keffi Metropolis, Nasarawa State Nigeria, International Journal of Agricultural Sciences, 2(2): 109-112.
  5. Thrustfield VM. Veterinary epidemiology. 3rd Edition, Blackwell Science Ltd, Oxford,UK. 2005; 183.
Full Text Read : 1448 Downloads : 260
Previous Next

Open Access Policy

Close