A study was conducted to assess the organoleptic and microbiological quality of pizzas sold in various outlets in and around Greater Hyderabad Municipal Corporation Telangana State, India. The sensory scores were higher for reputed brands, least for street vendors and in between for moderate fast food shops. The total viable count was highest (8.8×106CFU/gm) in burgers from street vendors, lowest (3.0×105CFU/gm) from reputed brands, whereas it is intermediate (3.56×106CFU/gm) from moderate fast food centers. The coliforms count and faecal coliform in the burgers collected from reputed brands, moderate fast food centers and street vendors was4.0×103 and 1.2×10^3,4.2×104 and 2.1×104and 4.8×104 and 9.8×103CFU/gm respectively. The yeast and moulds counts were 1.9×104, 2.6×105and 2.0×106CFU/gm in samples from reputed brands, moderate fast food centres and street vendors respectively. The incidence and counts of Staphylococcus spp., E. coli, Salmonella spp., Vibrio spp. and Listeria spp. were also noted.
Changes in life style has changed food consumption resulting introduction of western food products and fast foods into the human life in India. Bakery products consumption has been increased recently and the quality of these products is doubtful. Their shelf life is limited due to microbial spoilage. Pizza and Italian products has sprung into Indian market which has stolen the hearts of youth and its market has been expanded in a steady manner. Pizza base is made from dough and its quality is questionable in the market due to use of contaminated ingredients. The cheese used, as a layer on the base is one of the major source for contamination of pizza as the industry may acquire inferior quality of cheese from market at cheaper price. The sauce, prepared locally is generally used in pizza preparation, which also contribute some amount of contamination.
The materials used for toppings i.e., cabbage, tomato, mushroom, spinach etc., whose quality from the market is double. Apart from the ingredients, processing will also play an important role causing survival of existing pathogens. Nowadays many small-scale operators entered into western products and the contamination after processing is huge due to unhygienic conditions during storage and distribution. Even though the quality from multinational and national companies is satisfactory but the products from middle and lower level of outlets is highly questionable. Presently very scanty work has been published on the acceptability and microbiological quality of pizzas available in the Indian market, so this work was undertaken to assess the sensory evaluation and microbiological quality of pizza available in different types of outlets.
Material and Methods
30 pizza samples each from reputed brands, moderate fast food centers and street vendors were collected from in and around Greater Hyderabad Municipal Corporation (GHMC). Samples were collected aseptically in sterilized plastic pouches with self-sealing, kept in ice box and immediately transported to laboratory.
Preparation of Dilutions
About 9 ml distilled water in test tubes were serially arranged and sterilized in autoclave. 1 gram of sample was mixed with first test tube of distilled water, which makes 1:10 dilution. 1 ml of this is transferred to second and so on to make serial dilutions of 1:100, 1:1000, 1:10000, 1:100000 and 1:1000000.
Preparation of Media
Nutrient agar (TVC), MacConckey (Coliforms), Potato Dextrose Agar (Yeast and Mould), Xylose lysine Deoxycholate Agar (Salmonella), Eosin Methylene Blue Agar (E coli), Mannitol Salt Agar (Staphylococcus), Thiosulfate-Citrate-Bilesalts –Sucrose agar (Vibrio), Listeria Selective Agar Base (Listeria) were prepared and sterilized. 1ml of dilutions serially from 1:100000 to repeat factor for total viable count, 1:10 to 1:10000 for coliforms and specified dilutions for different microorganisms were transferred to petridishes and respective liquid media in sufficient quantity were added and allowed to set. Petridishes for TVC, coliforms and other pathogens were incubated at 35℃ for 24 to 48 hrs, whereas for faecal coliforms were incubated at 44.5℃ for 24 to 48 hrs and counts were made with the help of colony counter. The incubation period for yeast and moulds was 3-5 days. The pathogenic microorganisms are confirmed with various biochemical tests.
Results and Discussion
The packed and stored pizza samples were assessed for sensory attributes by trained panel of judges for appearance, flavor, body and texture and overall acceptability. The sensory attributes were estimated using five point Hedonic scale ranging from 1 to 5, 5-Excellent, 4- Very good, 3- Good, 2- Fair and1-Poor.A mean score of 2.5 and above is considered acceptable and below 2.5 needs to be evaluated for microbiological quality. The sensory scores where higher for reputed brands, least for street vendors and in between for moderate fast food shops. The pizza samples, which were unsold, might have been carried over in street vendor samples, which might have been the reason for low sensory scores. The sensory evaluation of pizza samples are presented in Table 1.
Table 1: Sensory Evaluation of Pizza samples from different sources.
Score/ Character Reputed brands Moderate fast Food shops Street vendors
Appearance 4.28±0.12 3.86±0.25 3.02±0.25
Flavour 4.52±0.15 4.21±0.22 3.52±0.22
Body 4.28±0.52 4.01±0.18 3.28±0.28
Overall Acceptability 4.48±0.28 4.01±0.22 2.62±0.28
The total viable count was highest (8.8×106CFU/gm) in pizza samples from street vendors, lowest (3.0×105CFU/gm) from reputed brands, whereas it is intermediate (3.56×106CFU/gm) from moderate fast food centres. Eromo et al. (2016) reported total viable counts of 6.3×104 CFU/gm from street level samples which were less than the samples from different sources in the present study. A count of 2.0×103 CFU/gm in pizza samples was reported by Faruk and Akhter (2011) which was less than the counts from the street vendors. Singh et al. (2012) reported6.65logCFU/gm in unbaked pizza, which was less than the counts from street vendors in the present study. The count of 3.5 ×106 CFU/gm in the samples from moderate fast food centres was almost similar to the counts of 8.8×106 CFU/gm observed by Kneifel and Berger (1994) in pizza mixture. High counts of 10-109CFU/gm, 3×108CFU/gm, 1.78×104CFU/gm were reported by Saifullah et al. (2015), Sengul (2006), Machala (1961) in pizza, pizza cheese and pizza samples respectively, which were higher than the levels of total viable counts observed in the present study in the samples from any of the three sources. The low counts in pizza samples collected from reputed brands might be due to the hygienic practices followed by the handlers (Dickson, 1987). The Total Viable Count, Coliform count and Yeast and Mould counts are presented in Table 2.
Table 2: Total viable count, Coliform count, Faecalcoliform count and Yeast and Mould counts in pizza samples from different sources (CFU/gm)
Source TVC Coliforms Faecal Coliforms Yeast and Mould
Reputed brands 3.0×105 4.0×103 1.2×10^3 1.9×104
Moderate fast food centers 3.56×106 4.2×104 9.8×103 2.6×105
Street vendors 8.8×106 4.8×104 2.1×104 2.0×106
The coliforms count in the pizza samples collected from reputed brands, moderate fast food centres and street vendors were 4.0×103, 4.2×104 and 4.8×104 CFU/gm respectively. Eromo et al. (2016) reported coliform counts of 1.4×103 CFU/gm in pizza samples which were slightly less than the counts in the present study from street vendors. Machala (1961), Faruk and Akhter (2011) and Saifullah et al. (2015), reported coliform counts of Nil, 1.85×102 and 101 CFU/gm in pizza samples, which were less than the coliform counts from reputed brands in the present study, whereas higher counts of 4.2×104 CFU/gm in Turkish cheese were reported by Sengul (2006). The Faecal coliform counts was 1.2×10^3,9.8×10^3and 2.1×10^4 CFU/gmin the samples from reputed brands, moderate fast food centres and street vendors respectively in the present study. Faruk and Akhter (2011) reported faecal coliforms counts of 15 CFU/gm in pizza samples, which were less than the samples from any of the three sources in the present study.
The Yeast and Mould counts was 1.9×104, 2.6×105 and 2.0×106CFU/gm in the samples from reputed brands, moderate fast food centres and street vendors respectively. High counts of 2.1×106 CFU/gm were reported by Sengul (2006) in pizza cheese samples, which were higher than the samples from any source in the present study. Pinho and Furlong (2006) reported higher counts of 2.9×106CFU/gm in precooked pizza samples.
Incidence of Pathogens
The incidence of Staphylococcus spp. in the Pizza samples was very high from all the sources in the present study i.e., 90%, 93.33% and 100% from reputed brands, moderate fast food centres and street vendors respectively. An incidence of 13.6% and 3% in hamburger patties and meatballs were reported by Aycicek et al. (2005) which were far less than incidence observed in the present study in the pizza samples from all the three sources. Low incidence of 3%, 6.4%,12% in pizza samples, hamburger patties, vegetable salad than the incidence from all the three sources in the present study was reported by Gutiérrez et al. (2012). The incidence of different pathogens in the burger samples in present study are presented in Table 3.
Table 3: Incidence of different pathogens in pizza samples from different sources
Source Staphylococcus spp. E.coli Salmonella spp. Vibrio spp. Listeria spp.
Reputed brands 27 (90%) 15 (50%) 19(63.3%) 6 (20%) 6 (20%)
Moderate fast foods 28 (93.3%) 24 (80%) 24 (80%) 13 (43.3%) 7 (23.33%)
Street vendors 30 (100%) 30 (100%) 27 (90%) 16 (53.33%) 12 (40%)
The incidence of E. coli in burger samples was 100% from street vendors followed by moderate fast food centres (80%) and least from reputed brands (50%) in present study. An incidence of 0.8% in fully cooked food samples was reported by Balzaretti and Marzano (2013), which was far less than the incidence observed in the present study in the pizza samples from all the three sources, whereas no incidence was reported by Pinho and Furlong (2000). The incidence of Salmonella spp. in pizza samples in the present study was least from reputed brands (63.33%), high from street vendors (90%) and in between (80%) from moderate fast food centres. Very low incidence of salmonella (1.56 and 12.9%) was observed in uncooked and cooked products by El-Shrek et al. (2012).
The incidence of Vibrio spp. was 20%, 43.33% and 53.33% in the pizza samples from reputed brands, moderate fast food centres and street vendors respectively in the present study. Azwai et al. (2016) reported an incidence of 50% and 46.6% from uncooked and cooked meat sample respectively, which was almost similar to the incidence in the samples from moderate fast food centres. Jaksic et al. (2002) reported an incidence of 19.6% which was almost similar to the incidence in present study from reputed brands, whereas very low level of incidence (1.6%) was reported by Ripabelli et al. (1999). The incidence of Listeria spp. from reputed brands, moderate fast food centres and street vendors was 20%,23.33% and 40% respectively in the present study. High incidence of 78%in ready to eat soft cheese samples was reported by Oyinloye (2016),56.3% and 86.4% in chicken legs and ground meat were reported byFarber et al. (1989) respectively, than the samples collected from all the three sources in the present study. An incidence of 6.5% in meat and meat products was reported by Jalali and Abedi (2008) which was far less than the incidence from the samples collected from any sources in the present study.
Pathogenic Microorganisms Counts
The counts of Staphylococcus spp. in the pizza samples from reputed brands, moderate fast food centres and street vendors were6.12×103,8.38×104,3.63×106 CFU/gm respectively. Counts of 3.12 log CFU/gm, 3.0-4.3 log CFU/gm, 2.6 log CFU/gm and 2.8-3.5 log CFU/gm were reported by Aycicek et al. (2005),Gonul et al.(1996), Dickson(1987) and Gutiérrez et al. (2012) respectively in Russian salad , hamburger patties, pizzas samples respectively, which were less than the counts observed from reputed brands in the present study. Counts of 10.94×103,8.98x103and2.17×103 CFU/gm were reported by El-Sherif et al. (1983), Essa and Makar (2004) and Saleh et al. (2010) respectively which were almost similar to the counts observed from reputed brands in the present study. The counts of the pathogenic microorganisms in pizza samples from different sources are presented in Table 4. The E. coli counts in the present study were1.82×102, 8.56×103and 4.68×105CFU/gm from reputed brands, moderate fast food centres and street vendors respectively. Counts of 3.37logCFU/gm from frozen pizza samples and 1.5-2.6log CFU/gmin frozen salami were reported by Dickson(1987) and Faith et al.(1997) respectively, which were less than the counts observed from the reputed brands in the present study.
Source Staphylococcus spp. E.coli Salmonella spp. Vibrio spp Listeria spp.
Reputed brands 6.12×103 1.82×102 3.26×103 1.1×101 1.28×101
Moderate fast food centers 8.38×104 8.56×103 5.6×103 2.8×102 2.86×102
Street vendors 3.63×106 4.68×105 8.38×104 8.68×103 1.28×103
Table 4: Pathogenic microorganisms counts in pizza samples from different sources (CFU/gm).
The Salmonella spp. counts were3.26×103,5.6×103 and 8.38×104CFU/gm in the pizza samples from reputed brands, moderate fast food centres and street vendors respectively. Dickson (1987), observed counts of 3.6-4.5log CFU/gm in frozen pizza samples, which were, less than the counts observed from the reputed brands in the present study. Balzaretti and Marzano (2013) reported nil counts of salmonella spp. in pizza samples.
The Vibrio spp. counts of 1.1×101,2.8×102and 8.68×103CFU/gm were from reputed brands, moderate fast food centres and street vendors respectively, in the present study. Higher counts of 4.2×105,4.2×107, 6.1×106and 4.6×106in tomatoes, chicken samples, pizza and burgers samples were reported by Mrityunjoy et al. (2013) which are higher than the counts in the samples from street vendors in the present study. Counts of 1.1×103in frozen meat samples were reported by Mrityunjoy et al. (2013) which were similar to the counts from the samples from moderate fast food centres in the present study. The Listeria spp. counts were1.28×101, 2.86×102 and 1.28×103CFU/gm from reputed brands, moderate fast food centres and street vendors respectively in the present study. Counts of 3.0-4.3 log CFU/gm were reported by Ünalan et al.(2013) which were higher than the counts from three sources in the present study. Any food items sold under strict personal and environmental hygienic conditions will have low incidence and counts of pathogens. This might have reflected higher counts in the pizza samples sold by street vendors and minimum counts in the pizza samples sold by reputed brands. The hygienic conditions maintained in the retailers of moderate fast food centers were better than street vendors and less than that of reputed brands and so the incidence as well as pathogenic counts were moderate.
The microbiological counts, incidence and counts of pathogenic microorganisms are optimum in burger samples from reputed brands but very high counts in the samples from moderate fast food centres and street vendors. Unless strict hygienic measures are taken at these sources, public health problems will arise.
Aycicek, H., Cakiroglu, S and Stevenson, T. H (2005). Incidence of Staphylococcus aureus in ready-to-eat meals from military cafeterias in Ankara, Turkey. Food control, 16(6), 531-534.
Azwai, S. M., Alfallani, E. A., Abolghait, S. K., Garbaj, A. M., Naas, H. T., Moawad, A. A andEldaghayes, I. M (2016). Isolation and molecular identification of Vibrio spp. By sequencing of 16S rdna from seafood, meat and meat products in Libya. Open veterinary journal, 6(1), 36-43.
Balzaretti, C. M and Marzano, M. A (2013). Prevention of travel-related foodborne diseases: Microbiological risk assessment of food handlers and ready-to-eat foods in northern Italy airport restaurants. Food control, 29(1), 202-207.
Çetinkaya, F and Soyutemiz, G. E (2004). A study on survival of Listeria monocytogenes during manufacture and ripening of Kashar cheese. Turkish Journal of Veterinary and Animal Sciences, 28(5), 927-932.
Dickson, J. S (1987). Survival of selected indicator and pathogenic bacteria in refrigerated pizzas. Journal of Food Protection, 50(10), 859-861.
El-Dosoky, H. F. A., shafik, S and Baher, M.Detetion of spoilage and food poisoning bacteria in some ready to eat meat products in dakahlia governorate.
El-Sherif, A. M., Khalafalla, F. A and Darwish, A. M (1991). Microflora in beef burger sandwiches. Journal of Egypt Vet Med Ass, 51, 169-177.
Eromo, T., Tassew, H., Daka, D and Kibru, G (2016). Bacteriological quality of street foods and antimicrobial resistance of isolates in Hawassa, Ethiopia. Ethiopian journal of health sciences, 26(6), 533-542.
Essa, H. H and Makar, N. H (2003). Bacteriological quality of beef burgers in Assiut City, Assiut. Vet. Medical Journal, 49, 81-88.
Faith, N. G., Parniere, N., Larson, T.,Lorang, T. D., Kaspar, C. W and Luchansky, J. B (1998). Viability of Escherichia coli O157: H7 in salami following conditioning of batter, fermentation and drying of sticks, and storage of slices. Journal of food protection, 61(4), 377-382.
Farber, J. M., Sanders, G. W and Johnston, M. A (1989). A survey of various foods for the presence of Listeria species. Journal of food protection, 52(7), 456-458.
Faruk, M. O and Akhter, M. Z (2011). Presence of coliforms and fecal coliforms in fast food items of local restaurants and fast food outlets of Dhaka city. Bangladesh Journal of Microbiology, 28(1), 49-51.
GönüL, Ş. A., Karapinar, M and Karagözlü, N (1996). Microbiological quality control of delicatessen food products. Turkish Journal of Biology, 20(3), 263-271.
Gutiérrez, D., Delgado, S., Vázquez-Sánchez, D., Martínez, B., Cabo, M. L., Rodríguez, A and García, P (2012). Incidence of Staphylococcus aureus and analysis of associated bacterial communities on food industry surfaces. Appl. Environ. Microbiol., 78(24), 8547-8554.
Jakšić, S., Uhitil, S., Petrak, T., Bažulić, D and Karolyi, L.G(2002). Occurrence of Vibrio spp. In sea fish, shrimps and bivalve molluscs harvested from Adriatic Sea. Food Control, 13(8), 491-493.
Jalali, M and Abedi, D (2008). Prevalence of Listeria species in food products in Isfahan, Iran. International journal of food microbiology, 122(3), 336-340.
Kneifel, W and Berger, E (1994). Microbiological criteria of random samples of spices and herbs retailed on the Austrian market. Journal of Food Protection, 57(10), 893-901.
Machala, W. E (1961). A bacteriological investigation of frozen foods in the Oklahoma City area. Journal of Milk and Food Technology, 24(10), 323-327.
Mrityunjoy, A., Kaniz, F., Fahmida, J., Shanzida, J. S., Aftab, U. M and Rashed, N (2013). Prevalence of Vibrio cholerae in different food samples in the city of Dhaka, Bangladesh. International Food Research Journal, 20(2), 1017.
Oyinloye, J. M. A (2016). Detection and Molecular Characterization of Listeria species in ‘Wara’, A West African Local Cheese Sold in Ekiti State. International Journal of Current Microbiology and Applied Sciences, 5, 941-948.
Pinho, B. H and Furlong, E. B (2000). The occurrence of molds, yeasts and mycotoxins in pre-cooked pizza dough sold in Southern Rio Grande do Sul. Brazilian Journal of Microbiology, 31(2), 99-102.
Ripabelli, G., Sammarco, M. L., Grasso, G. M., Fanelli, I., Caprioli, A andLuzzi, I (1999). Occurrence of Vibrio and other pathogenic bacteria in Mytilusgalloprovincialis (mussels) harvested from Adriatic Sea, Italy. International Journal of Food Microbiology, 49(1-2), 43-48.
Saif-ullah, M. R., Mehboob, K., Ahmad, M. U. D., Rafique, R., Mushtaq, M. H., Sharif, F and Parveen, S (2015). Microbiological Analysis of Different Snack Foods as Public Health Significance. Infectious Disease Journal of Pakistan, 24(1).
Saleh, E. A., Ali, H. A and Abu-Khadra, A. M (2010). Detection of some food poisoning microorganisms in some meat products. Alexandria Journal of Veterinary Sciences, 31(1), 27-33.
Şengül, M (2006). Microbiological characterization of Civil cheese, a traditional Turkish cheese: microbiological quality, isolation and identification of its indigenous Lactobacilli. World Journal of Microbiology and Biotechnology, 22(6), 613-618.
Singh, P., Wani, A.A and Goyal, G.K (2012). Shelf-life extension of fresh ready-to-bake pizza by the application of modified atmosphere packaging. Food and bioprocess technology, 5(3), 1028-1037.
Ünalan, İ. U., Arcan, İ., Korel, F and Yemenicioğlu, A (2013). Application of active zein-based films with controlled release properties to control Listeria monocytogenes growth and lipid oxidation in fresh Kashar cheese. Innovative Food Science & Emerging Technologies, 20, 208-214.
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