Colibacillosis is an economically important disease, causing neonatal diarrhoea and septicaemia in calves. The present study was carried out in the Department of Veterinary Epidemiology and Preventive Medicine. A total of thirty neonatal calves affected with diarrhoea were selected from Wayanad district. The present study was conducted to identify colibacillosis in calves from Wayanad district by identification of clinical signs such as diarrhoea, pyrexia, severe dehydration, etc., Faecal sample culture in MacConkey’s agar and Faecal culture in EMB agar. To identify the level of metabolic acidosis bicarbonate level of venous blood was analyzed using Epoc® Blood Gas Electrolyte Analyzer. Significant increase in pCO2, lactate and creatinine were observed. significant reduction in pH, pO2, base excess (BE), Na+, K+, Glucose, Bicarbonate and chloride were significantly lower in test group comparing with control group (p≤ 0.01). There was no significant difference between test group and control group in Ca+ levels.