The objective of this study was to isolate Escherichia coli spp. and to identify the risk factors associated with subclinical mastitis in goats. The overall animal wise prevalence of Escherichia coli SCM was 9.13 per cent and udder halves wise 6.02 per cent. Out of 1312 udder halves, 7.1 per cent udder halves were unilaterally affected and the prevalence was higher in right udder halves (6.38 per cent). The breed wise prevalence of Escherichia coli SCM was highest (25.0 per cent) in Black Bengal goats and the age wise prevalence of Escherichia coli SCM was found highest (23.80 per cent) in lactating goats in 3-4 years of age. The lactation / parity wise prevalence of Escherichia coli SCM was observed highest (18.08 per cent) in 3rd lactation. The lactation stage wise prevalence of SCM was found highest (17.43 per cent) in early lactation stage. The antibiogram assay revealed that Escherichia coli spp. was most susceptible to enrofloxacin with 23.25 mm zone of inhibition.