Crossbred cattle (Jersey x Sahiwal), eight year of age was suffering from a clinical mastitis. It was treated with cephalosporin according to antibiotic sensitivity test. The Candida species was isolated in pure form and it was subjected to five antifungal drugs viz: Ketaconazole, Fluconazole, Clo-trimazole, Itraconazole and Polymixin-B. The Polymyxin B was the best effective drug against candida mastitis. Indiscriminate utilization of antibiotics without knowing the cause of infection could be a reason behind the candida mastitis.