The present study was planned to find out the skin thickness and its relationship with milk yield in Murrah buffaloes. The experiment was conducted at ABIS Buffalo Dairy Farm, Rajnandgaon on 300 milking Murrah buffaloes. The data was statistically analyzed using R statistical software. The average skin thickness of neck, chest, abdomen, rump, hind quarter, udder and flank regions were 6.27 ± 0.11, 6.13 ± 0.13, 7.28 ± 0.14, 9.78 ± 0.15, 8.00 ± 0.12, 4.14 ± 0.09 and 8.53 ± 0.12 mm respectively. In this study the skin fold thickness of neck and chest region had highly significant effect, whereas skin thickness of other regions under study had no significant effect on total lactation milk yield. The study indicated that except udder region the correlation of all skin thickness with TMY was found to be negative and significant. The regression coefficient indicated that an increase in skin thickness by 1 mm at different places would cause a decrease in average total lactation milk yield up to 135 liters. These results could facilitate the use of skin fold thickness in selection of better animals under field conditions.