The present study was conducted to detect the alterations of serum biochemical profile of cattle infected with tick- borne haemoparasitic diseases i.e. theileriosis, Babesiosis and anaplasmosis etc. A total of 310 cattle from 7 different districts and 2 cattle farms of West Bengal were screened randomly for the presence of haemoparasites from July, 2015 to June, 2016. Serum samples from both infected and healthy cattle were collected for estimation of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase, bilirubin, creatinine, blood urea nitrogen (BUN), glucose, calcium and phosphorus using spectrophotometer. Serum biochemistry of infected cattle revealed significantly increased levels of AST, ALT, alkaline phosphatase (ALP), bilirubin, creatinine and BUN. Serum glucose and mineral levels were found significantly decreased when compared with healthy group. Serum biochemical alterations should be considered in order to achieve prognosis and initiation of appropriate therapeutic regimen for a favourable outcome of the tick-borne haemoparasitic diseases.