Serum trace minerals viz. Zinc (Zn), Iron (Fe), Copper (Cu) and Manganese (Mn) level were estimated in Pati, Khaki Campbell and Nageswari ducks of Assam and compared the concentration between healthy and those suffered with duck plague. Significant (P≤ 0.05) reduction in the serum Zn, Fe and Mn in all varieties of ducks during duck plague outbreak. A non-significant decrease in the serum Cu level was also observed during duck plague outbreak. From the present study, it can be concluded that significantly (P<0.05) lower level of Zn, Fe and Mn may act as a contributing factor for duck plague in different varieties of duck.