Assessment of knowledge level of farmers is the perquisite of implementation of any development programme. Therefore, the present study was designed to assess existing knowledge level of the farmers from purposively selected five aspirational districts of West Bengal as identified by the NITI Aayog, Govt. of India. Respondents were randomly selected from one block of each district with total sample size of 4285 number of farmers for the present study. The data was collected with the help of pre-tested structured interview schedule. It was found Knowledge Score among the farmers of Nadia district (37.37±3.55) was highest and in South Dinajpur (30.16±3.03) was lowest than the other three districts viz Birbhum (35.07±2.85), Malda (34.18±3.09) and Murshidabad (33.51±3.15). Study also revealed that the farmers of Nadia district were having more knowledge about improved animal husbandry practices.