Cardiotoxicity Induced by Doxorubicin in Wistar Rats and its Amelioration with Ascorbic Acid and Spirulina

Y. Ravi Kumar; D. Madhuri; A. Gopala Reddy; A. Anadkumar; B. Anil  Kumar; T. Chandravathi

Authors

Y. Ravi Kumar Department of Veterinary Pathology, PVNRTVU, Hyderabad-500030, INDIA
D. Madhuri Department of Veterinary Pathology, PVNRTVU, Hyderabad-500030, INDIA
A. Gopala Reddy Department of Veterinary Pharmacology & Toxicology, PVNRTVU, Hyderabad-500030, INDIA
A. Anadkumar College of Veterinary Science, SVVU, Andhra Pradesh-516360, INDIA
B. Anil Kumar Department of Veterinary Pharmacology & Toxicology, PVNRTVU, Hyderabad-500030, INDIA
T. Chandravathi Department of Veterinary Pathology, PVNRTVU, Hyderabad-500030, INDIA

Keywords:

Amelioration, Ascorbic Acid, Cardiotoxicity, Doxorubicin, Spirulina, Wistar Rats

Abstract

The study was designed to evaluate the cardiotoxicity of Doxorubicin in Wistar rats and amelioration by ascorbic acid and spirulina. Female rats were divided into four groups. Animals were slaughtered at the end of the experiments (28^th day) and collected blood for analysis. The biochemical assays showed significant (P<0.05) increase in creatinine phosphokinase (CPK), lactate dehydrogenase (LDH), cardiac troponins (cTn) in Doxorubicin treated rats. There was considerable amelioration in ascorbic acid and spirulina groups. Grossly, heart revealed severe congestion, endocardial haemorrhages. Histopathological changes in heart of doxorubicin group revealed severe disruption, separation, degeneration, necrosis, fragmentation of muscle fibers with endocardial haemorrhages, edema, and focal infiltration of lymphocytes. The ameliorative groups 3 and 4 showed mild to moderate improvement. The tissue enzymes assays revealed a significant (P<0.05) increase in TBARS and significant (P<0.05) decrease of GSH and SOD activities in group 2. The ameliorative groups 3 and 4 showed mild to moderate improvement.