Antimicrobial Susceptibility of Pseudomonas Spp. Isolated from Dogs with Otitis Externa

Yen Tran; Huy Do; Duyen Nguyen; An Vo

Authors

Tran Thi Kim Yen Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Viet Nam
Do Huynh Duc Huy Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Viet Nam
Nguyen Tran Hao Duyen Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Viet Nam
Vo Thi Tra An Faculty of Animal Science and Veterinary Medicine, Nong Lam University HCMC, Viet Nam

Keywords:

Dogs, Otitis Externa, Pseudomonas spp

Abstract

In dogs, Pseudomonas spp. is among the most common bacterial pathogens that cause otitis. Choosing the correct antibiotic for treating dog ear infections will increase treatment success and reduce the possibility of antibiotic resistance. Therefore, this study was performed to determine the antibiotic resistance profile of Pseudomonas spp. isolated from dogs suffering from otitis externa (OE). Seventy-three sterile swab samples were collected from the terminal part of the vertical ear canals of 54 dogs suffering from OE in Ho Chi Minh City, Vietnam. Samples were tested for cytology, bacterial culture, biochemical tests, and antimicrobial susceptibility testing. Twelve Pseudomonas isolates were collected and tested for antimicrobial susceptibility against 10 antibiotics. The Pseudomonas isolates were fully sensitive to amikacin, tobramycin, gentamicin, and ceftazidime. Most of them were sensitive to polymyxin B, enrofloxacin, marbofloxacin, and ciprofloxacin. Meanwhile, the majority of the collection was resistant to tetracycline and doxycycline. Thus, we recommend using antibiotics such as amikacin, tobramycin, gentamicin or polymyxin B, and fluoroquinolone antibiotics to treat canine ear infections caused by Pseudomonas spp.

References

Angus, J. C., Lichtensteiger, C., Campbell, K. L., & Schaeffer, D. J. (2002). Breed variations in histopathologic features of chronic severe otitis externa in dogs: 80 cases (1995–2001). Journal of the American Veterinary Medical Association, 221(7), 1000–1006.

Boda, C., P. Liege & C. A. Reme. (2011). Evaluation of owner compliance with topical treatment of acute otitis externa in dogs: A comparative study of two auricular formulations. International Journal of Applied Research in Veterinary Medicine., 9, 157–165.

Brame, B., Cain, C. (2021). Chronic otitis in cats: clinical management of primary, predisposing and perpetuating factors. Journal of Feline Medicine and Surgery, 2021, 433 446. 10.1177/1098612X211007072

Cole, L. K. (2004). Otoscopic evaluation of the ear canal. Veterinary Clinics of North America: Small Animal Practice, 34(2), 397–410.

CLSI. Performance standards for antimicrobial susceptibility testing . 31st ed. CLSI guideline M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2021.

Hillier, A., Alcorn, J. R., Cole, L. K., & Kowalski, J. J. (2006). Pyoderma caused by Pseudomonas aeruginosa infection in dogs: 20 cases. Veterinary Dermatology, 17(6), 432–439.

Hung, P. (2009). Survey on ear infections in dogs and treatment effectiveness at Pet-pro veterinary clinic at 389 Cong Hoa, Ward 13, Tan Binh, Ho Chi Minh City. Veterinary Profession.

Martin Barrasa, J. L., Lupiola Gomez, P., Gonzalez Lama, Z., & Tejedor Junco, M. T. (2000). Antibacterial Susceptibility Patterns of Pseudomonas Strains Isolated from Chronic Canine Otitis Externa. Journal of Veterinary

Medicine Series B, 47(3), 191–196.

Mekic, S., Matanović, K., & Šeol, B. (2011). Antimicrobial susceptibility of Pseudomonas aeruginosa isolates from dogs with otitis externa. Veterinary Record, 169(5), 125.

Morris, D. O. (2004). Medical therapy of otitis externa and otitis media. Veterinary Clinics of North America: Small Animal Practice, 34(2), 541–555.

Rougier, S., Borell, D., Pheulpin, S., Woehrle, F., & Boisrame, B. (2005). A comparative study of two antimicrobial/anti-inflammatory formulations in the treatment of canine otitis externa. Veterinary Dermatology, 16(5), 299–307.

Wallace KA, DeHeer HL, Patel RT. (2013). The external ear canal. Diagnostic Cytology and Hematology of the Dog and Cat. 4th ed. St. Louis, MO: Elsevier Mosby, 171-179.

Zamankhan Malayeri, H., Jamshidi, S., & Zahraei Salehi, T. (2010). Identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs. Veterinary Research Communications, 34(5), 435–444.