A Novel Approach In Treating Teat Wound Using Autologous Peripheral Blood Mononuclear Cells (PBMC) In A Six Cows – A Clinical Study

N. Gurunathan; Vigneswari Murugesan; S. Tina Roshini; N. Arul Jothi; C. Chimithi; N. Arrivukkarasi

Authors

N. Gurunathan Assistant Professor, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009.
Vigneswari Murugesan Assistant Professor, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009.
S. Tina Roshini Assistant Professor, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009.
N. Arul Jothi Professor and Head, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009
C. Chimithi Post Graduate Scholar, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009
N. Arrivukkarasi Post Graduate Scholar, Department of Veterinary Surgery and Radiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry, India - 605009

Keywords:

Peripheral Blood Mononuclear Cells, Teat wound

Abstract

A total of six cows with teat wounds with fistula or intact teat cistern were subjected to the present study. After clinical examination, all the animals were sedated with inj. xylazine @ 0.1 mg/kg b.wt. I/V and ring block with 2% Lignocaine hydrochloride, the wound edges were debrided and irrigated with 0.5% povidone-iodine diluted with normal saline. The patency of the teat canal was maintained by placing infant feeding tube size No 10 in-situ. A three-layer suturing pattern was followed. The mucosal layer and muscle layer were closed using simple continuous suture pattern with polyglactin 910 size 3-0. The skin was apposed using disposable stainless steel skin staples. Whole blood was collected and processed to extract Peripheral Blood Mononuclear Cells (PBMC) which were injected subcutaneously around the surgical site. Therapeutic phonophoresis @ 1watt/cm2 was performed for 5 minutes around the peri-wound area on the day of surgery and the 10th postoperative day. All the animals were administered with intramuscular Inj. Streptopenicillin @ 10mg/kg b. wt and inj. Meloxicam @ 0.5 mg/kg b. wt, postoperatively. Inj. Metrinidazole 10ml with 5 ml of Inj. Gentamicin was given as an intra-mammary infusion for 5 days. Postoperatively the surgical site was protected with an adhesive bandage (Dynafix). Wound dressing was done on the 7th and staples were removed on the 10th postoperative day. Morphological and Ultrasonographic evaluation of healing was carried out on the 10th postoperative day. PBMC favoured early uncomplicated scarless wound healing which can prevent mastitis in milking cows, and well accepted by the animals.

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