EFFECT OF PURSLANE (Portulaca Oleracea) LEAVES EXTRACT IN TRIS EGG YOLK CITRATE EXTENDER ON OXYDATIVE STRESS PARAMETERS OF CRYOPRESERVED SURTI BUCK SEMEN

Abstract

The aim of this research was to investigate the antioxidant properties of Purslane (Portulaca Oleracea) leaves extract in the Tris egg yolk citrate extender on the cryopreserved Surti buck semen. A total of 64 semen samples were collected from four Surti bucks, with 16 samples from each buck, twice a week using artificial vagina method. The semen samples were diluted with the Tris egg yolk citrate extender and the Purslane (Portulaca Oleracea) leaves aqueous extract was added at different concentrations, namely 0% (T1), 1% (T2), 2% (T3), and 3% (T4), to maintain the final concentration of 100×10⁶ sperm/ml with a pH of 6.5-6.8. The levels of lipid peroxidation and reduced glutathione were evaluated at three stages: just after dilution (initial), pre-freeze, and post-thaw (24 hours after cryopreservation) stages. Results showed that Lipid peroxidation (MDA) decreased with an increase in Purslane (Portulaca Oleracea) leaves aqueous extract concentration at initial, pre-freeze, and post-thaw stages, with the lowest level observed in the 3% Purslane (Portulaca Oleracea) leaves aqueous extract group. Additionally, Glutathione (GSH) values increased with an increase in Purslane (Portulaca Oleracea) leaves aqueous extract concentration at the initial, pre-freeze, and post-thaw stages, with the highest GSH activity observed in the 3% Purslane (Portulaca Oleracea) leaves aqueous extract in tris egg yolk citrate extender group. These results indicate that Purslane (Portulaca Oleracea) leaves aqueous extract is an effective antioxidant that enhances the sperm antioxidant defense in cryopreserved Surti buck semen.