Molecular Survey of Enteric Viruses Associated with Poultry Enteritis in Southern Rajasthan, India

Authors

  • A. Saraswat Department of Veterinary Microbiology, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan, INDIA
  • D. K. Sharma Department of Veterinary Microbiology, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan, INDIA
  • B. Joseph Department of Veterinary Microbiology and Biotechnology, PGIVER, Jaipur, Rajasthan, INDIA
  • R. Singathia Department of Veterinary Microbiology, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan, INDIA
  • C. Patidar Department of Veterinary Microbiology, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200831062702%20%20%20%20%20

Keywords:

Avian coronavirus, Chicken parvovirus, Chicken astrovirus, Enteric Viruses, Fowl adenovirus, Poultry Enteritis

Abstract

Enteric viruses play major role in affecting the poultry industry worldwide, the occurrence of enteric viruses in poultry is still very poorly explored in India. Therefore, a molecular survey has been performed to determine the presence of enteric viruses among enteritis affected poultry flocks of Udaipur and Chittorgarh districts of Southern Rajasthan, India. Total 60 pooled samples of intestine were collected from enteritis affected dead birds, DNA and RNA viruses were detected by PCR and reverse transcriptase PCR respectively. Enteric viruses namely Fowl adenovirus-I(FADV-I), Chicken parvovirus (CPV), Avian coronavirus (ACV), and Chicken astroviruses (CAstV) were detected in 73.32 % of investigated samples. FAdV-I (21.66%) was detected as highest single virus infection followed by ACV (13.33%). Combinations of two or more enteric viruses were simultaneously identified in 38.33% samples.

References

Cavanagh, D., Mawditt, K., Welchman, D.D.B., Britton, P. and Gough, R.E. (2002). Corona viruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys. Avian Pathology,31, 81–93.

De la Torre, D. I., Nuñez, L. F., Astolfi-Ferreira, C. S. and Piantino Ferreira, A. J. (2018). Enteric virus diversity examined by molecular methods in brazilian poultry flocks. Veterinary Science, 5(2), 38.

Decaesstecker, M., Charlierand, G. and Meulemans, G. (1988). Epidemiological study of enteric viruses in broiler chickens: Comparison of tissue culture and direct electron microscopy. Avian Pathology, 17(2), 477-486.

El-Behery, H. N., Awad, S. S. and Kasem, S. G. (2016). Molecular characterization of infectious bronchitis virus in chicken. Egyptian Journal of Veterinary Science,47(2), 133-149.

Hess, M. (2000). Detection and differentiation of avian adenoviruses: a review. Avian Pathology, 29(3), 195-206.

Hewson, K. A., O'Rourke, D. and Noormohammadi, A. H. (2010). Detection of avian nephritis virus in Australian chicken flocks. Avian diseases, 54(3), 990-993.

Jindal, N., Patnayak, D. P., Chander, Y., Ziegler, A. F. and Goyal, S. M. (2010). Detection and molecular characterization of enteric viruses in breeder turkeys. Avian Pathology, 39(1), 53-61.

Kaithal, B., Jindal, N., Kumar, P. and Mor, S. K., (2016). Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India. Actavirologica, 60(4), 361-371.

Koo, B. S., Lee, H. R., Jeon, E. O., Han, M. S., Min, K. C., Lee, S. B. and Mo, I. P. (2013). Molecular survey of enteric viruses in commercial chicken farms in Korea with a history of enteritis. Poultry Science, 92(11), 2876-2885.

Mahmood, Z. H., Sleman, R. R. and Uthman, A. U. (2011). Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Veterinary Microbiology, 150(1-2), 21-27.

Mettifogo, E., Nuñez, L. F., Chacón, J. L., Santander Parra, S. H., Astolfi-Ferreira, C. S., Jerez, J. A., and Piantino Ferreira, A. J. (2014). Emergence of enteric viruses in production chickens is a concern for avian health. The Scientific World Journal, 2014. doi: 10.1155/2014/450423. eCollection 2014.

Meulemans, G., Boschmans, M., Van den Berg, T. P. and Decaesstecker, M. (2001). Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathology, 30(6), 655-660.

Michael Day, J., Spackman, E., & Pantin-Jackwood, M. (2007). A multiplex RT-PCR test for the differential identification of turkey astrovirus type 1, turkey astrovirus type 2, chicken astrovirus, avian nephritis virus, and avian rotavirus. Avian diseases, 51(3), 681-684.

Moura-Alvarez, J., Chacon, J. V., Scanavini, L. S., Nunez, L. F. N., Astolfi-Ferreira, C. S., Jones, R. C. and Piantino Ferreira, A. J. (2013). Enteric viruses in Brazilian turkey flocks: single and multiple virus infection frequency according to age and clinical signs of intestinal disease. Poultry Science, 92(4), 945-955.

Niu, Y., Sun, Q., Zhang, G., Sun, W., Liu, X., Xiao, Y. and Liu, S. (2018). Epidemiological investigation of outbreaks of fowl adenovirus infections in commercial chickens in China. Transboundary and Emerging Diseases, 65(1), 121-126.

Palade, E.A., Kisary, J., Benyeda, Z., Mandoki, M., Balka, G., Jakab, C., Végh, B., Demeter and Z. Rusvai M. (2011). Naturally occurring parvoviral infection in Hungarian broiler flocks. Avian Pathology, (40), 191-197

Pantin-Jackwood, M. J., Michael Day, J., Jackwood, M. W. and Spackman, E. (2008). Enteric viruses detected by molecular methods in commercial chicken and turkey flocks in the United States between 2005 and 2006. Avian diseases, 52(2), 235-244.

Roussan, D., Shaheen, I., Khawaldeh, G., Totanji, W. and Al-Rifai, R. (2012). Simultaneous detection of astrovirus, rotavirus, reovirus and adenovirus type 1 in broiler chicken flocks. Polish Journal of Veterinary Science, 15(2), 337-344.

Smyth, V. J., Jewhurst, H. L., Adair, B. M. and Todd, D. (2009). Detection of chicken astrovirus by reverse transcriptase-polymerase chain reaction. Avian Pathology, 38(4), 293-299.

Yegani, M.and Korver, D. (2008). Factors affecting intestinal health in poultry. Poultry Science, (87), 2052-2063.

Zsak, L., Cha, R. M. and Michael Day, J. (2012). Chicken parvovirus–Induced runting-stunting syndrome in young broilers. Avian Diseases, 57(1), 123-127.

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Published

31-01-2021

How to Cite

Saraswat, A., Sharma, D. K., Joseph, B., Singathia, R., & Patidar, C. (2021). Molecular Survey of Enteric Viruses Associated with Poultry Enteritis in Southern Rajasthan, India. International Journal of Livestock Research, 11(1), 75–81. https://doi.org/10.5455/ijlr.20200831062702

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