In vitro and Extracellular Assays Based Scoring System for Selection of Potential Probiotic Isolates with Antioxidant Property from Native Chicken


  • Divya Manjari Karnan Ph.D Scholar, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, INDIA
  • M. Parthiban Professor and Head, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, INDIA
  • T. M. A. Senthilkumar Project Director, Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies, TANUVAS, Chennai, INDIA
  • R. Karunakaran Professor and Head, Department of Animal Nutrition, Madras Veterinary College, TANUVAS, Chennai, INDIA
  • V. Appa Rao Dean, College of Food and Dairy Technology, TANUVAS, Chennai, INDIA



Antioxidant, Broiler, In Vitro Assays, Native Chicken, Probiotics, Real Time PCR


Probiotics have been used as a major alternative approach in the newly hatched chicks to colonize normal micro flora in the intestine. This study involves the characterization of antioxidant potential of probiotic isolates obtained from backyard chicken. Different probiotic isolates namely E. thailandicus, E. hirae, E. faecium, E. fecalis, E. durans, P. acidilactici, L. salivarius were identified by molecular methods using PCR amplification followed by sequencing. Extracellular assays viz DPPH and FRAP assays were performed for assessing the antioxidant potential of these isolates. In vitro assay was also performed in Caco2 and HepG2 cell lines to study the antioxidant changes induced by the probiotic isolates by profiling the antioxidant genes such as SOD (Superoxide dismutase) and GPx (Glutathione peroxidase) with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) as control by real time PCR. An intricate scoring system was established for evaluating the probiotic isolates for their efficient antioxidant activity. The scoring system revealed that the isolates E. hirae-1, E. faecium-1, E. fecalis-3, P. acidilactici exhibited maximum potential with respect to antioxidant activity.


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How to Cite

Karnan, D. M., Parthiban, M., Senthilkumar, T. M. A., Karunakaran, R., & Appa Rao, V. (2021). In vitro and Extracellular Assays Based Scoring System for Selection of Potential Probiotic Isolates with Antioxidant Property from Native Chicken. International Journal of Livestock Research, 11(1), 138–144.

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