In vitro and Extracellular Assays Based Scoring System for Selection of Potential Probiotic Isolates with Antioxidant Property from Native Chicken
DOI:
https://doi.org/10.5455/ijlr.20200920050734Keywords:
Antioxidant, Broiler, In Vitro Assays, Native Chicken, Probiotics, Real Time PCRAbstract
Probiotics have been used as a major alternative approach in the newly hatched chicks to colonize normal micro flora in the intestine. This study involves the characterization of antioxidant potential of probiotic isolates obtained from backyard chicken. Different probiotic isolates namely E. thailandicus, E. hirae, E. faecium, E. fecalis, E. durans, P. acidilactici, L. salivarius were identified by molecular methods using PCR amplification followed by sequencing. Extracellular assays viz DPPH and FRAP assays were performed for assessing the antioxidant potential of these isolates. In vitro assay was also performed in Caco2 and HepG2 cell lines to study the antioxidant changes induced by the probiotic isolates by profiling the antioxidant genes such as SOD (Superoxide dismutase) and GPx (Glutathione peroxidase) with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) as control by real time PCR. An intricate scoring system was established for evaluating the probiotic isolates for their efficient antioxidant activity. The scoring system revealed that the isolates E. hirae-1, E. faecium-1, E. fecalis-3, P. acidilactici exhibited maximum potential with respect to antioxidant activity.References
AFRC, R. F. (1989). Probiotics in man and animals. Journal of Applied Bacteriology, 66(5), 365-378.
Brolazo, E. M., Leite, D. S., Tiba, M. R., Villarroel, M., Marconi, C., & Simoes, J. A. (2011). Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates. Brazilian Journal of Microbiology, 42(1), 225-232.
Charteris, W. P., Kelly, P. M., Morelli, L., & Collins, J. K. (1998). Ingredient selection criteria for probiotic microorganisms in functional dairy foods. International Journal of Dairy Technology, 51(4), 123-136.
deMelo Pereira, G. V., de Oliveira Coelho, B., Júnior, A. I. M., Thomaz-Soccol, V., & Soccol, C. R. (2018). How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances, 36(8), 2060-2076.
Dhama, K., Verma, V., Sawant, P. M., Tiwari, R., Vaid, R. K., & Chauhan, R. S. (2011). Applications of probiotics in poultry: Enhancing immunity and beneficial effects on production performances and health-A review. Journal of Immunology and Immunopathology, 13(1), 1-19.
Drago, L., Nicola, L., Iemoli, E., Banfi, G., & De Vecchi, E. (2010). Strain-dependent release of cytokines modulated by Lactobacillus salivarius human isolates in an in vitro model. BMC Research Notes, 3(1), 1-5.
Figueroa‐González, I., Quijano, G., Ramirez, G., & Cruz‐Guerrero, A. (2011). Probiotics and prebiotics—perspectives and challenges. Journal of the Science of Food and Agriculture, 91(8), 1341-1348.
Han, Q., Kong, B., Chen, Q., Sun, F., & Zhang, H. (2017). In vitro comparison of probiotic properties of lactic acid bacteria isolated from Harbin dry sausages and selected probiotics. Journal of Functional Foods, 32, 391-400.
Huang, Y., & Adams, M. C. (2004). In vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propioni bacteria. International Journal of Food Microbiology, 91(3), 253-260.
Kabir, S. (2009). Effect of probiotics on broiler meat quality. African Journal of Biotechnology, 8(15).
Kailasapathy, K., & Chin, J. (2000). Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunology and Cell Biology, 78(1), 80-88.
Liska, D. J. (1998). The detoxification enzyme systems. Altern Med Rev, 3(3), 187-198.
Manjari, K. D., Divya, G., Saravanan, T. S., & Parthiban, M. (2019). In vitro selection of potential immunomodulatory multispecies lactic acid bacteria in broilers. Indian Journal of Animal Research, 53(5), 655-660.
Maragkoudakis, P. A., Zoumpopoulou, G., Miaris, C., Kalantzopoulos, G., Pot, B., & Tsakalidou, E. (2006). Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal, 16(3), 189-199.
Panda, A. K., Rama Rao, S. S., Raju, M. V., & Sharma, S. S. (2008). Effect of probiotic (Lactobacillus sporogenes) feeding on egg production and quality, yolk cholesterol and humoral immune response of White Leghorn layer breeders. Journal of the Science of Food and Agriculture, 88(1), 43-47.
Settivari, R. S., Bhusari, S., Evans, T., Eichen, P. A., Hearne, L. B., Antoniou, E., &Spiers, D. E. (2006). Genomic analysis of the impact of fescue toxicosis on hepatic function. Journal of Animal Science, 84(5), 1279-1294.
Surai, P. F. (2002). Natural antioxidants in avian nutrition and reproduction (pp. 5-9). Nottingham: Nottingham University Press.
Walter, J., Tannock, G. W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D. M., Munro, K., & Alatossava, T. (2000). Detection and identification of gastrointestinal lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Applied and Environmental Microbiology, 66(1), 297-303.
Wang, Y., Wu, Y., Wang, Y., Xu, H., Mei, X., Yu, D., & Li, W. (2017). Antioxidant properties of probiotic bacteria. Nutrients, 9(5), 521.
Xing, J., Wang, G., Zhang, Q., Liu, X., Gu, Z., Zhang, H., & Chen, W. (2015). Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods. PLoS One, 10(3), e0119058.
Yarru, L. P., Settivari, R. S., Gowda, N. K. S., Antoniou, E., Ledoux, D. R., & Rottinghaus, G. E. (2009). Effects of turmeric (Curcuma longa) on the expression of hepatic genes associated with biotransformation, antioxidant, and immune systems in broiler chicks fed aflatoxin. Poultry Science, 88(12), 2620-2627.
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