Study on Therapeutic Management of Tick-Borne Diseases in Cross-Bred Cattle

Vishnu Rai; Arbind Singh; Prem Sagar Maurya; Ramakant; Deepika Singh; Vikas Jaiswal; Desh Deepak; Kuldeep Tyagi; Amit Verma

Study on Therapeutic Management of Tick-Borne Diseases in Cross-Bred Cattle

Authors

Vishnu Rai MVsc Scholar, Veterinary Medicine,
Arbind Singh Assistant Professor, Livestock Farm Complex,
Prem Sagar Maurya Associate Professor, Veterinary Parasitology,
Ramakant PhD Scholar, Veterinary Medicine,
Deepika Singh MVsc Scholar, Veterinary Medicine,
Vikas Jaiswal Assistant Professor, Veterinary Pathology, https://orcid.org/0000-0001-5457-3419
Desh Deepak Assistant Professor, Veterinary Medicine,
Kuldeep Tyagi Professor & Head, Animal Genetics & Breeding,
Amit Verma Professor & Head, Veterinary Medicine, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh -250110, INDIA

Keywords:

Anaplasmosis, cattle, Buparvaquone, Giemsa stain, Oxytetracyclin, Theileriosis

Abstract

Tick-borne hemoprotozoan diseases such as Anaplasmosis and Theileriosis, caused by obligate intra-erythrocytic parasites result in significant economic losses for the livestock industry worldwide. In the current study, 40 cross-bred cattle with tick infestations were examined showing clinical signs such as weakness, inappetence, pyrexia, pale mucous membranes, anemia, lymph node swelling, and nasal discharge. Haematological analysis revealed anaemia, low erythrocyte count, and decreased packed cell volume. Peripheral thin blood smear showed the presence of Anaplasma spp. (03/40), Theileria spp. (08/40), and co-infection of Theileria spp. with Anaplasma spp. (08/40) in erythrocytes as well as Koch’s Blue Bodies in lymphocytes. On the first day, cases of anaplasmosis (n=03) were treated with Oxytetracycline Long acting @ 22 mg/kg b.wt. intramuscularly, theileriosis cases (n=08) were treated with Inj. Buparvaquone @ 2.5 mg/kg b.wt. intramuscularly and mixed infections (n=08) were treated with both drugs. All clinical cases also received symptomatic treatment such as Flunixin meglumine @ 1.1–2.2 mg/kg b.wt. and liver tonic (Inj. Rumeric 10 ml). On the seventh day only two animals showed theileria infection and were treated again with Buparvaquone. It can be concluded that 100% of bovine anaplasmosis cases recovered with one dose of oxytetracycline, while 87.5% of bovine tropical theileriosis cases recovered after one dose of buparvaquone, and 100% of cases recovered after two doses of Buparvaquone. Anaplasmosis and Theileriosis co-infections were prevalent in the cattle of this region and can be successfully treated with the above therapeutic management.

References

Allsopp, B.A., Baylis, H.A., Allsopp, M.T., Cavalier Smith, T., Bishop, R.P., Carrington, D.M., Sohanpal, B. & Spooner, P. (1993). Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitology, 107, 157–165.

Altay, K., Aydin, M. F., Dumanli, N. & Aktas, M. (2008). Molecular detection of Theileria and Babesia infections in cattle. Veterinary parasitology, 158(4), 295-301.

Anonymous, (1993). Tropical Animal Health and Production. India.

Baek, B. K., Choi, I. H., Kim, B. S., Hansen, R. & Kakoma, I. (1992a). Immunogenicity and protective efficacy of solubilized merozoite enriched Theileria sergentiimmunogens. I: Protection against homologous stabilate challenge. Kisaengchunghak Chapchi, 30, 133–140.

Baek, B. K., Kim, B. S., Choi, I. H., Yang, K. C., Hansen, R. and Kakoma, I. (1992b). Immunogenicity and protective efficacy of solubilized merozoite enriched Theileria sergentiimmunogens. II: Protection against natural exposure under field conditions. Kisaengchunghak Chapchi, 30, 201–208.

Bishop, R. P., Sohanpal, B. K. & Morzaria, S. P. (1994). Cloning and characterization of a repetitive DNA sequence from Theileria mutans: application as a species-specific probe. Parasitology Research, 80, 33–41.

Ceci, L. E. Kirvar, G. Carelli, D. Brown, Sasanelli, M. & Sparagano, O. (1997). Evidence of Theileria buffeli infection in cattle in southern Italy. Veterinary Record, 140, 581–583.

Chae, J., Lee, J. O., Kwon, P. J., Holman, Waghela, S. D. & Wagner, G.G. (1998). Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene variable (V4) region among and within geographic isolates of Theileria from cattle, elk and whitetailed deer. Veterinary Parasitology, 75: 41–52.

Chae, J., Levy, M., Hunt, J., Schlater, Jr. J., Snider, G., Waghela, S. D., Holman, P. J. & Wagner, G.G. (1999a). Theileria spp. infections associated with bovine fatalities in the United States confirmed by small subunit rRNA gene analyses of blood and tick samples. Journal of Clinical Microbiology, 37, 3037–3040.

Chandratre, G. A., Jangir, B. L., Sharma, M., Jakhar, K. K. & Kumar, T. (2018). Clinico-haematological alterations and therapeutic management of tick borne fever in cross bred cow. J. Parasit. Dis., 42(4), 643-645.

Coetzee, J. F., Kocan, K. M., Higgins, J. J., Apley, M. D. & Jones, D. E. (2009). Ultrastructural and fluorochromatic changes of Anaplasma marginale exposed to Oxyoxytetracycline, Imidocarb and Enrofloxacin in short-term erythrocyte cultures. Vet. Microbiol., 136, 45-53.

Constable, P. D., Hinchcliff, K. W., Done, S. H. & Grunberg, W. (2017). Veterinary Medicine: A textbook of diseases of cattle, horses, sheep, pigs and goats. 11th Ed. Elseveir.

De, U., Dey, S., Banerjee, P. & Sahoo, M. (2012). Correlations among Anaplasma marginale parasitemia and markers of oxidative stress in crossbred calves. Trop. Anim. Health Prod., 44(3), 385-388.

DeVos, A.J., Brock, R. and Molly, J.B. (2006). Tick borne diseases of cattle. In: Australian and New Zealand Standard Diagnostic Procedures. Sub Committee on Animal Health Laboratory Standards, pp 1–29. http://www.scahls.org.au/home.

Ganguly, A., Bisla, R. S., Singh, H., Bhanot, V., Kumar, A., Kumari, S. & Ganguly, I. (2017). Prevalence and haematobiochemical changes of tick borne haemoparasitic diseases in crossbred cattle of Haryana, India. Indian Journal of Animal Sciences, 87(5), 552-57.

George, N., Bhandari, V., Reddy, D.P. & Sharma, P. (2015). Molecular and Phylogenetic analysis revealed new genotypes of Theileria annulata parasites from India. Parasites & vectors, 8, 1-8.

Gubbels, M. J., d'Oliveira, C. & Jongejan, F. (2000). Development of an indirect Tams1 enzyme-linked immunosorbent assay for diagnosis of Theileria annulata infection in cattle. Clinical Diagnostic Laboratory Immunology, 7(3), 404-411.

Gubbels, M. J., Hong, Y., van der Weide, M. Qi. B., Nijman, I. J., Guangyuan, L. & Jongejan, F. (2000). Molecular characterisation of the Theileria buffeli/orientalis group. International journal for parasitology, 30(8), 943-952.

Gul, N., Ayaz, S., Gul, I., Adnan, M., Shams, S. & Akbar, N. (2015). Tropical Theileriosis and East Coast Fever in cattle: present, past and future perspective. Int. J. Curr. Microbiol. Appl. Sci., 4(8), 1000-1018.

Jaswal, H., Bal, M. S., Singla, L. D., Gupta, K. & Brar, A. P. (2015). Pathological observations on clinical Anaplasma marginale infection in cattle. J. Parasit. Dis. 39(3), 495-498.

Jeong, W., Yoon, S. H., An, D. J., Cho, S. H., Lee, K. K. & Kim, J. Y. (2010). A molecular phylogeny of the benign Theileria parasites based on major piroplasm surface protein (MPSP) gene sequences. Parasitology, 137(2), 241-249.

Kaur, R., Yadav, A., Rafiqi, S. I., Godara, R., Sudan, V., Chakraborty, D. & Katoch, R. (2021). Epidemiology, haematology and molecular characterization of haemoprotozoon and rickettsial organisms causing infections in cattle of Jammu region, North India. BMC veterinary research, 17(1), 219.

Kaur, R., Yadav, A., Rafiqi, S.I., Godara, R., Sudan, V., Chakraborty, D. & Katoch, R. (2021). Epidemiology, haematology and molecular characterization of haemoprotozoon and rickettsial organisms causing infections in cattle of Jammu region, North India. BMC Veterinary Research, 17(1), 1-12.

Khaki, Z., Yasini, S.P. & Jalali, S.M. (2018). A survey of biochemical and acute phase proteins changes in sheep experimentally infected with Anaplasma ovis. Parasitological research, 8(12), 565-570.

Kumar, A., Singh, A., Verma, A. K., Maurya, P. S., Kumar, A., Desh Deepak, Singh, R. & Sarkar, T.K. (2023b). Prevalence and associated risk factors of bovine anaplasmosis in western Uttar Pradesh. Veterinary Practitioner, 24(01), 39-41

Kumar, A., Singh, A., Verma, A. K., Maurya, P. S., Prajapati, M. R., Kumar, A. & Sarkar, T.K. (2023a). Phylogenetic analysis and molecular characterization of field isolates of Anaplasma spp. from cattle in India. Veterinarkshi archiv, 93(5), 535-548

Kumar, P. P. & Sangwan, A. K. (2010). Comparative prevalence of subclinical bovine Anaplasmosis under different cattle management systems in Haryana. Haryana Vet, 49(1), 1-5.

Lorusso, V., Picozzi, K., de Bronsvoort, B.M., Majekodunmi, A., Dongkum, C., Balak, G. & Welburn, S.C. (2013). Ixodid ticks of traditionally managed cattle in central Nigeria: where Rhipicephalus (Boophilus) microplus does not dare (yet?). Parasites & vectors, 6, 1-10.

Magona, J. W., Walubengo, J., Olaho-Mukani, W., Jonsson, N. N., Welburn, S. W. & Eisler, M.C. (2011). Spatial variation of tick abundance and seroconversion rates of indigenous cattle to Anaplasma marginale, Babesia bigemina and Theileria parva infections in Uganda. Experimental and Applied Acarology, 55, 203-213.

Mohammed, G., Saqib, M., Athar, M., Khan, M. & Asi, M.N. (1999). Clinico-epidemiological and therapeutic aspects of Bovine Theileriosis. Pakistan Vet. J., 19(2), 64-71.

Molad, T., Mazuz, M. L., Fleiderovitz, L., Fish, L., Savitsky, I., Krigel, Y. & Shkap, V. (2006). Molecular and serological detection of A. central and A. marginale infected cattle grazing within an endemic area. Veterinary microbiology, 113(1-2), 55-62.

Nasir, A. A. (2000). Effect of Theileriosis on blood parameters of exotic cattle and efficacy of Buparvaquone and Oxytetracycline. Pak. J. Bio. Sci., 3(6), 1027-1028.

Patil, N. A., Lakshmaiah, K. R. and Harapanahalli, M. D. (1995). Efficacy of Butalex (Buparvaquone) in the treatment of clinical cases of bovine tropical Theileriasis. Indian Journal of animal research, 29(1), 59-61.

Potgieter, F.T. & Stoltsz, W.H. (2004). Bovine Anaplasmosis. In: Coetzer JAW, Tustin RC, Infectious Diseases of Livestock, Oxford University Press, Cape Town, South Africa, 594–616.

Radiostits, O. M., Gray, C. C., Hinchelift, K. W. & Constable, P. D. (2007). Veterinary medicine. A textbook of the disease of cattle, horses. Sheep, Pigs and Goats, 10th edition. Sunders, London, 1576-1580.

Schalm, O. W., Jain, N. C. & Carrol, E. J. (1975). Veterinary Haematology 3rd edn. edited by OW Schalm, NC Jain and EJ Carroll, Lea and Febiger Publication, Philadelphia.

Scholar, E. M. & Pratt, W. B. (2000). Bacteriostatic inhibitors of protein synthesis: Oxytetracyclines. In: The Antimicrobial Drug. 2nd ed. New York: Oxford University Press; 184-199.

Subramanian, B., Vijayalakshmi, P., Das, S.S. & Selvi, D. (2018). Prevalence of Anaplasmosis Caused by Anaplasma marginale in Cattle in and around Puducherry Region, India. Int. J. Curr. Microbiol. App. Sci., 7(4), 189-193.

Sugimoto, C. & Fujisaki, K. (2002). Non-transforming Theileria parasites of ruminants. Theileria, 93-106.

Vahora, S. P., Patel, J. V., Patel, B. B., Patel, S. B. and Umale, R. H. (2012). Seasonal incidence of Haemoprotozoal diseases in crossbred cattle and buffalo in Kaira and Anand districts of Gujarat, India. Veterinary World, 5(4), 223.

Zakkyeh, T., Ali, O. M., Nasibeh, H. V., Reza, Y. E. M., Farhang, B. & Fatemeh, M. (2012). First molecular detection of Theileria ovis in Rhipicephalus sanguineus tick in Iran. Asian Pacific Journal of Tropical Medicine, 5(1), 29-32.

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Published

30-06-2024

How to Cite

Rai, V., Singh, A., Maurya, P. S., Ramakant, Singh, D., Jaiswal, V., … Verma, A. (2024). Study on Therapeutic Management of Tick-Borne Diseases in Cross-Bred Cattle . International Journal of Livestock Research, 14(6), 49–55. Retrieved from http://ijlr.org/ojs_journal/index.php/ijlr/article/view/423