Enzyme Immunoassay for Determination of Plasma Total Estrogen in Black Bengal Goat During Peripubertal Period

Authors

  • Avijit Haldar ICAR-Agricultural Technology Application Research Institute (ATARI), Indian Council of Agricultural Research, Bhumi Vihar Complex, Block- GB, Sector- III, Salt Lake, Kolkata- 700097, West Bengal, INDIA https://orcid.org/0000-0003-0770-3983
  • Aditi Patra Department of Animal Science, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, P.O. Krishi Viswavidyalaya, Mohanpur, Nadia- 741252, West Bengal, INDIA
  • Chanchal K. Biswas Department of Animal Science, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, P.O. Krishi Viswavidyalaya, Mohanpur, Nadia- 741252, West Bengal, INDIA
  • Bukkaaraya S. Prakash Indian Council of Agricultural Research, Krishi Bhavan, New Delhi- 110001, INDIA

DOI:

https://doi.org/10.5455/ijlr.20191125120939

Keywords:

Black Bengal, Enzyme Immunoassay, Goat, Peripubetal Period, Total Estrogen

Abstract

The current study aims the validation of an enzyme immunoassay (EIA) for the quantification of total
estrogen (TE) in blood plasma of Black Bengal goats during peripubertal period. A microtiter plate based
competitive assay was developed using a capture EIA for anti-bovine TE and horseradish peroxidase
coupled bovine TE. Bovine TE standards were prepared in charcoal-dextran stripped goat plasma.
Specificity: A dose-response inhibition curve resulting from serially diluted goat plasma was parallel to the
standard curve using bovine TE and thus it confirmed the similar specificity of bovine TE standard and
endogenous TE in goat plasma for bovine TE antibody. Sensitivity: The minimum detection level of the
assay was 0.10 pg TE/ 50 l, corresponding to 2 pg/ ml plasma. Precision: The inter- and intra-assay
coefficients of variations (for repeatability and reproducibility, respectively) for the pooled plasma samples
in 10 assays were 8.5% and 7.3% and 9.7% and 7.3%, respectively. Validation: Pubertal symptoms in goats
were coincided with the higher plasma TE level. Plasma TE levels in the goats exposed with buck were
higher (P<0.05) than the goats maintained without a buck.

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Published

31-03-2020

How to Cite

Haldar, A., Patra, A., Biswas, C. K., & Prakash, B. S. (2020). Enzyme Immunoassay for Determination of Plasma Total Estrogen in Black Bengal Goat During Peripubertal Period. International Journal of Livestock Research, 10(3), 160–175. https://doi.org/10.5455/ijlr.20191125120939

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