Enzyme Immunoassay for Determination of Plasma Total Estrogen in Black Bengal Goat During Peripubertal Period

Abstract

The current study aims the validation of an enzyme immunoassay (EIA) for the quantification of total
estrogen (TE) in blood plasma of Black Bengal goats during peripubertal period. A microtiter plate based
competitive assay was developed using a capture EIA for anti-bovine TE and horseradish peroxidase
coupled bovine TE. Bovine TE standards were prepared in charcoal-dextran stripped goat plasma.
Specificity: A dose-response inhibition curve resulting from serially diluted goat plasma was parallel to the
standard curve using bovine TE and thus it confirmed the similar specificity of bovine TE standard and
endogenous TE in goat plasma for bovine TE antibody. Sensitivity: The minimum detection level of the
assay was 0.10 pg TE/ 50 l, corresponding to 2 pg/ ml plasma. Precision: The inter- and intra-assay
coefficients of variations (for repeatability and reproducibility, respectively) for the pooled plasma samples
in 10 assays were 8.5% and 7.3% and 9.7% and 7.3%, respectively. Validation: Pubertal symptoms in goats
were coincided with the higher plasma TE level. Plasma TE levels in the goats exposed with buck were
higher (P<0.05) than the goats maintained without a buck.