Study of Plasmid Profiling of Clostridium perfringens Isolated from Small Ruminants of Odisha

Niranjan Soren Soren; Rajashree Mishra; Iswar Anand Senapati; Kailash Singh Bisht; Bidyut Parva Mishra; Pravas Ranjan  Sahoo

doi:10.5455/ijlr.20191126074300

Authors

Niranjan Soren MVSc, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA
Rajashree Mishra HOD, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA
Iswar Anand Senapati MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA
Kailash Singh Bisht MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA
Bidyut Parva Mishra Assistant Professor, Department of Livestock Products Technology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA
Pravas Ranjan Sahoo Assistant Professor, Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, INDIA

DOI:

https://doi.org/10.5455/ijlr.20191126074300%20%20%20%20%20

Keywords:

Clostridium perfringens, Plasmid Profiling, Phenol-Chloroform Method, Small Ruminant

Abstract

In the present study 209 intestinal and faecal samples of small ruminants were collected from different farms and localities in and around Bhubaneswar, Odisha. Out of which 159 samples were of goats and 50 samples of sheep, it includes both intestinal and faecal samples. The collected samples were processed for plasmid profiling of C. perfringens. A total of 19 samples were found positive for C. perfringens indicating the prevalence of 9.09% in small ruminant population of Odisha. In order to study the epidemiological strain variation, all the present isolates were subjected to plasmid profiling. Plasmid profiling of the local isolates is an important tool for strain related identification of disease in sheep and goat. In this study plasmid DNA yield was low, as for high yield large amount of fresh C. perfringens culture was needed, but an attempt has been carried out for plasmid curing. Effect of various parameters like different percentage of gel, running buffer and temperature were done and it was found that 0.7% agarose gel, TBE buffer at 30^oC of temperature were optimum condition for plasmid profiling. The present study showed that DNase activity remains a block in the plasmid isolation and profiling of Clostridial species. Therefore, need for a DNase inhibitor that may increase the efficacy of plasmid curing.