Isolation and Characterization of Cellulose Degrading Bacteria from Saliva of‘Jaffrabadi’Breed of Buffalo

Authors

  • Ashok A. Shinde MGMU Institute of Biosciences and Technology, MGM University, Cidco N-6, Aurangabad, Maharashtra, INDIA
  • Faiyaz K. Shaikh MGMU Institute of Biosciences and Technology, MGM University, Cidco N-6, Aurangabad, Maharashtra, INDIA
  • Ketali P. Pisal MGMU Institute of Biosciences and Technology, MGM University, Cidco N-6, Aurangabad, Maharashtra, INDIA
  • Pratiksha S. Patil MGMU Institute of Biosciences and Technology, MGM University, Cidco N-6, Aurangabad, Maharashtra, INDIA

DOI:

https://doi.org/10.5455/ijlr.20200509115503%20%20%20%20%20

Keywords:

Bacillus subtilis, Biotechnology, Cellulase, CMC agar, Ruminant, SDS-PAGE

Abstract

Microorganisms with cellulolytic activity are considered as potential candidates in the processing of cellulosic biomass for biotechnological applications. This study was conducted to isolate and characterize cellulose-degrading bacteria collected from the saliva of Jaffrabadi breed of buffalo by morphological and biochemical analysis. The cellulolytic bacteria were isolated and purified on CMC (Carboxy methyl cellulose) agar. Bacterial isolate produced a clear transparent zone when stained with Congo red, confirming cellulase activity. Morphological examination revealed Gram-positive, rod or bacilli- shaped, non-motile, and spore forming nature of bacterial isolate. Different biochemical tests suggested that bacterial isolate was Bacillus subtilis. The cellulase enzyme was partially purified by (NH4)2SO4 and studied using CMCase assay. The activity of partially purified cellulose was higher (8µg/ml/min) as compared to that of crude supernatant (5.5µg/ml/min). The optimum pH and optimum temperature range were found to be 6.5 - 7.5 and 40 50 °C. Protein profiling using 10% SDS-PAGE further confirmed the purity of the partially purified sample.

Downloads

Published

30-09-2020

How to Cite

Shinde, A. A., Shaikh, F. K., Pisal , K. P., & Patil, P. S. (2020). Isolation and Characterization of Cellulose Degrading Bacteria from Saliva of‘Jaffrabadi’Breed of Buffalo. International Journal of Livestock Research, 10(9), 96–104. https://doi.org/10.5455/ijlr.20200509115503

Similar Articles

1 2 3 4 > >> 

You may also start an advanced similarity search for this article.