Molecular Detection of Certain Virulence Genes of Avian Pathogenic Escherichia coli (APEC) in Chicken

Tarakeswara Rao M; Rama Devi V; Sreedevi C; Srinivasa Rao T; Sudha Rani Chowdary CH; Annapurna P

doi:10.5455/ijlr.20220601053400

Authors

M. Tarakeswara Rao Veterinary Assistant Surgeon
V. Rama Devi Professor, Department of Veterinary Pathology, NTR CVSc, Gannavaram
C. Sreedevi Professor, Department of Veterinary Parasitology, NTR CVSc, Gannavaram
T. Srinivasa Rao 4. Professor, Department of Veterinary Public Health and Epidemiology, NTR CVSc, Gannavaram
CH. Sudha Rani Chowdary Assistant Professor, Department of Veterinary Pathology, NTR CVSc, Gannavaram
P.Annapurna Associate Professor (Rtd.), Department of Veterinary Pathology, NTR CVSc, Gannavaram

DOI:

https://doi.org/10.5455/ijlr.20220601053400

Keywords:

Escherichia coli, APEC, virulence genes, chicken

Abstract

In the present study, 86 avian pathogenic E. coli isolates from chicken were subjected to multiplex PCR to detect the presence of virulence genes stx1, stx2, eaeA, and hlyA. Forty-seven isolates harbored one or more virulence genes, stx1, stx2, and eaeA while gene hlyA was not detected in any of the samples. Of these, 15 (17.44%) isolates harbored stx1gene, 11 (12.79%) isolates carried stx2 gene, 18 (20.93%) isolates were positive for both stx1 and stx2 genes and 3 (3.49%) isolates carried eaeA gene.

Author Biography

M. Tarakeswara Rao, Veterinary Assistant Surgeon

Veterinary Dispensary, Bapirajugudem

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