Present study was carried out on 40 repeat-breeding (RB) crossbred cows to assess the efficacy of a different treatment regimes on pregnancy rate. Cows were randomly assigned to four treatment groups. Group I cows (n = 10) were injected with 1500 IU of hCG at the time of AI, Group II cows (n = 10) were injected with 30 IU of oxytocin at the time of AI, Group III cows (n = 10) were treated with 30 ml of 10% Dextrose I/U two hours before the insemination and Group IV cows (n = 10) did not receive any treatment and served as control. Pregnancy status was diagnosed by uterine palpation at 45 to 60 days of post AI. Pregnancy rates, in group I to IV were 60%, 40%, 70% and 30% respectively. On average, cows treated with different protocols had higher pregnancy rate than control group. However, treatments with intra uterine Dextrose (10%, 30ml) showed higher pregnancy rate (70%) compared to treatment with in hCG (60%) and oxytocin (40%) in repeat breeder cows. Present study concludes that intra uterine flushing with Dextrose (10%, 30ml) two hours before AI showed higher pregnancy rate compared to other treatment regime indicating uterine environment highly determines reproductive efficiency of repeat breeding cow.