Thirty goats that were investigated for ruminal acidosis, with the history of accidental ingestion of carbohydrate rich diet, inappetence to anorexia, suspended rumination and clinical manifestations of distended rumen and diarrhoea were selected and were divided based on ruminal fluid pH and severity of ruminal acidosis into three different groups consisting of 10 goats in each. Ruminal fluid analysis was undertaken and compared against ten apparently healthy goats (group IV) from the same area with similar husbandry practices which served as healthy control. Basing on ruminal fluid pH, these goats were divided as mild acidosis (pH=5.5 - 6.5), moderate acidosis (pH = 4.5 - 5.5), and severe acidosis (pH = 4.0 - 4.5) group I, II and III respectively. Ruminal fluid parameters such as Methylene Blue Reduction Time (MBRT), Sedimentation activity time (SAT) and protozoal dead count significantly increased in group I. While, Ruminal acidotic goats had decreased rumen fluid parameters such as rumen fluid pH, protozoal motility, density, and iodophilic activity in group I. Rumen fluid color was grey in group I and milky grey in both group II and III, consistency was semisolid or slightly watery in group I and watery in both group II and III, odour was acidic in both group I and II and pungent acidic in group III of acidotic goats. Complete absence of protozoa in rumen fluid of both group II and III, and replacement of rumen fluid gram negative bacteria by gram positive bacteria in all acidotic goats. Based on study of all above parameters, the goats were confirmed as suffering from ruminal acidosis.