A Comparative Study of The Immunofluorescence in Brain, Salivary Gland and Cornea in Rabid Carcasses

Divya Chandran; Vijayan N.; N. Divakaran  Nair; Suvaneeth P.

Authors

Divya Chandran Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, INDIA
Vijayan N. Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, INDIA
N. Divakaran Nair Professor (Retd), Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, INDIA
Suvaneeth P. Assistant Professor, Department of Veterinary Pathology, Faculty of Veterinary and Animal Sciences, Banaras Hindu University, INDIA

Keywords:

Animals, Brain, Cornea, dFAT, Immunofluorescence, Rabies, Salivary Gland

Abstract

The present study has compared the immunofluorescence observed in the brain, cornea, and salivary gland of rabid carcasses. It has enabled a relative assessment of the reliability, sensitivity, and effectiveness of using corneal and salivary gland impression smears for the diagnosis of rabies in live animals. The study was conducted on 352 carcasses belonging to various species of domestic and wild animals. Direct Fluorescent Antibody Test (dFAT) was performed on the brain of carcasses to detect rabies. The smear from the cornea and salivary gland of rabies-positive animals were also subjected to dFAT and a comparison was made between each with regard to the intensity of staining and the area of distribution as indicated by fluorescence. The study revealed that the sensitivity of corneal impression smear for rabies diagnosis in animals was found to be lesser when compared with impression smear from the salivary gland.

References

Awahan, S., Sandhu, B.S., singh, C.K., Kaw, A. and Sood, N.K. 2011.Corneal impression smear and salivary smear tests for ante-mortem detection of rabies by immunofluorescence. Indian. Vet. J. 88(12):93-95.

Baer, M. 1991. The natural history of rabies (2nd Ed.) CRC Press. 535p.

Bourhy, H., Rollin, P.E., Vincent, J. and Sureau, P.1989. Comparative field evaluation of the fluorescent antibody test, virus isolation from tissue culture and enzyme immunodiagnosis for rabies. J. Clin. Microbiol. 27(3):519-523. DOI: 10.1128/jcm.27.3.519-523.1989

Crepin, P., Audry, L., Rotivel, Y., Gacoin, A., Caroff, C. and Bourhy, H. 1998. Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J. Clin. Microbiol. 36:1117-1121. DOI: 10.1128/JCM.36.4.1117-1121.1998

Dean, D., Abelseth, M. and Atanasiu, P. 1996. The fluorescent antibody test. In: Meslin, F. X., Kaplan, M. M. and Koprowski, H. editors. Laboratory techniques in rabies. 4th ed. Geneva, Switzerland: World Health Organization; 1996. p. 88-95.

Debbie, J.G. and Trimarchi, C.V. 1970. Pantropism of Rabies Virus in Free-Ranging Rabid Red Fox Vulpes fulva. J. Wildl. Dis. 6:500-506. DOI: 10.7589/0090-3558-6.4.500

Dierks, R.E., Murphy, F.A. and Harrison, A.K. 1969. Extraneural rabies virus infection.Virus development in fox salivary gland. Am. J. Path. 54(2): 251-274.

Fekadu, M., Shaddock, J.H. and Baer, G.M. 1981. Intermittent excretion of rabies virus in the saliva of a dog two and six months after it had recovered from experimental rabies. Am. J. Trop. Med. Hyg.30(5):1113-5.

Goldwasser, R.A., Kissling, R.E., Carski, T.R. and Hosty, T.S. 1959. Fluorescent antibody staining of rabies virus antigens in the salivary glands of rabid animals.Wld. Hlth. Org. 20:579-588.

Larghi, O.P, Gonztlez, L.E and Held, J.R. 1973. Evaluation of the corneal test as a laboratory method for rabies diagnosis. Appl. Micrbiol. 25:187.

Meslin, F.X. 1996. Laboratory techniques in rabies. (4th Ed.) World Health Organisation, Geneva.

Mukaka, M.M. 2012. A guide to appropriate use of Correlation coefficient in medical research. Malawi Med J. 24(3):69-71.

Parikh, R., Mathai, A., Parikh, S., Sekhar, G.C. and Thomas, R. 2008. Understanding and using sensitivity, specificity and predictive values. Indian J. Ophthalmol. 56(1):45-50. DOI: 10.4103/0301-4738.37595

Rupprecht, C.E., Fooks, A.R. and Abela-Ridder, B. 2018. Laboratory techniques in rabies Vol 1. (5th Ed.). World Health Organization, Geneva, Switzerland, pp. 108-123.

Tierkel, E.S. 1973. Rapid microscopic examination for Negri bodies and preparation of specimens for biological test. In: Kaplan, M.M. and Koprowski, H. (eds.), Laboratory Techniques in Rabies. (3rdEd.). World Health Organization, Geneva, pp. 41-50.

Warrell, D.A., Davidson, N.M., Pope, H.M., Bailie, W.E., Lawrie, J.H., Ormerod, L.D., Kertesz, A. and Lewis, P. 1976. Pathophysiologic studies in human rabies. Am J Med. 60(2):180-90. doi: 10.1016/0002-9343(76)90427-7. PMID: 766622.

Zaidman, G.W. and Billingsley, A. 1998.Corneal impression test for the diagnosis of acute rabies encephalitis. Ophthalmology. 105(2):249-251. DOI: 10.1016/S0161-6420(98)92860-3.