Study of FecXB and FecXG Mutations in a Group of Prolific Osmanabadi Goats

Abstract

Study was planned on FecX^B and FecX^G genes’ (Alleles of BMP15 gene exon II region) polymorphism in unrelated prolific Osmanabadi goat breed (n=60) of Maharashtra with triplets (n=42), quadruplets (n=16) and above kidding (n=2) and amplified 153 bp of FecX^B and a 141 bp of FecX^G genes were digested with DdeI and HinfI restriction enzymes, respectively. After DdeI and HinfI digestion of FecX^B and FecX^G found two fragments (122 and 31 bp) and (112 and 29 bp), respectively. All studied goats were found monomorphic for FecX^B and FecX^G genes. Previously reported sheep mutations hadn’t shown effect on prolificacy in studied goats. Species-specific differences in BMP15 may be associated with the differences in ovulation rate amongst species and hence, BMP15 gene may not be major gene associated with prolificacy/fecundity of Osmanabadi goats, therefore this study suggested to target other loci of FecX^B, FecX^G genes etc. in Osmanabadi goat and with larger samples.